水生生物学报Issue(1)：87-91,5.DOI:10.7541/2014.11
样品制备过程对测定水中溶解态微囊藻毒素的影响
THE INFLUENCES OF SAMPLE PREPARATION PROCESSES ON THE DETERMINATION OF DISSOLVED MICROCYSTINS IN FRESHWATERS
摘要
Abstract
Several processing protocols were systematically investigated for the preparation of dissolved microcystins (MCs) samples. Some key parameters affecting toxin determinations in sample preparation process were further identi-fied and quantified. We evaluate the influence of five different filters on the preparation of MCs samples. Results indi-cated that nearly 79% of added MCs could be absorbed by some membranes like CA filter for the filtration, which lead to the significant underestimation of MCs risks associated with toxin contaminations. Meanwhile, GF/C filter and PES filter tip are suitable for the preparation of dissolved MCs samples. In addition, centrifugation with the aim of removing algal cells also has significant impact on dissolved MCs determination. Results from this study will provide important information on the determination of dissolved microcystins and further lead to the standardization of the extracellular toxin determinations in risk assessments.关键词
溶解态微囊藻毒素/滤膜/测定/样品制备Key words
Dissolved microcystins/Filter membranes/Determination/Sample preparation分类
资源环境引用本文复制引用
贾云璐,陈伟,赵爽,周起超,宋立荣..样品制备过程对测定水中溶解态微囊藻毒素的影响[J].水生生物学报,2014,(1):87-91,5.基金项目
国家高技术研究发展计划(863计划)项目课题(编号:2009AA063005) （863计划）
国家自然科学基金项目(编号:40971249)资助 （编号:40971249）
