水生生物学报Issue(1)：142-149,8.DOI:10.7541/2014.19
斑马鱼ca15b的克隆及在原始生殖细胞中的特异表达
CLONING, IDENTIFICATION AND EXPRESSION ANALYSIS OFCA15B, A NOVEL GENE SPECIFICALLY EXPRESSED IN PRIMORDIAL GERM CELLS OF ZEBRAFISH
摘要
Abstract
Primordial germ cells (PGCs) are the ancestor cells of adult gametes at embryonic stage. PGCs-specific gene expression plays a fundamental role in germ cell induction, development, migration and differentiation. Understanding the functional role of PGCs-specifically expressed genes could provide us some tools to perform gene and embryo ma-nipulations. Screening a public database of zebrafish (http://www.zfin.org) we found a PGCs-specific candidate gene, carbonic anhydrase 15b (ca15b). To confirm this, whole-mountin situ hybridization and other technologies were uti-lized to analyze the expression ofca15bduring zebrafish early development. We observed thatca15b is a maternally expressed gene and it is highly expressed in germ plasm during cleavage stage. The expression ofca15b at mRNA level was specifically detected in the PGCs at blastula stage. At gastrula stage,ca15b is still strictly expressed in PGCs while its expression is undetectable in somatic cells. This specific expression pattern ofca15b persisted until one day post-fertilization. More importantly, the post-transcriptional regulation elements that mediate PGCs-specific expression ofca15b may locate in its coding sequence.关键词
斑马鱼/原始生殖细胞/表达模式/ca15bKey words
Zebrafish/Primordial germ cells/Expression pattern/ca15b分类
生物科学引用本文复制引用
杨明宇,王厚鹏,朱作言,孙永华..斑马鱼ca15b的克隆及在原始生殖细胞中的特异表达[J].水生生物学报,2014,(1):142-149,8.基金项目
国家科技部973计划课题(2010CB126306) （2010CB126306）
国家自然科学基金优秀青年科学基金(31222052)资助 （31222052）
