水生态学杂志Issue(2)：74-79,6.
基于卵黄蛋白原基因表达的水丝蚓促熟南美白对虾研究
Feasibility of Ripening Litopenaeus vannmei by Limnodrilus hoffmeisteri Based on Vitellogenin Gene Expression
摘要
Abstract
Traditional broodstock diets , being susceptible to virus attacks or carrying the virus to parent shrimps , bring a huge bio-security risk to shrimp breeding .This study was carried out to determine the feasibility of Limnod-rilus hoffmeisteri as a replacement of traditional bait to accelerate the maturity of parent shrimps of Litopenaeus van-namei, which was verified by the dynamics of vitellogenin gene expression in hepatopancreas and ovary of Litope-naeus vannmei.The parent shrimps fed on four different diets ( commercial parent shrimps feed , Limnodrilus hoffmeisteri combination bait , Limnodrilus hoffmeisteri and Nereis succinea combination bait ) , and the effect of each diet on the maturity of parent shrimps was evaluated by testing the gonad weight increase rate , eggs amount , aver-age spawning time and levels of VTG gene expression .The results showed that Limnodrilus hoffmeisteri, as a closely related fresh water species of Nereis succinea, had the best effect on maturation of parent shrimps .The highest go-nad weight increase rate of 1475%and the largest consecutive spawning rate with average value of 72 .08% were both in groups fed on Limnodrilus hoffmeisteri, with no significant difference from the groups fed on Nereis succinea combination bait .The dynamics of VTG mRNA level were observed in hepatopancreas and ovary of Litopenaeus van-nmei with the trend of increasing firstly and then decreasing , which reflected reproductive performance of the parent shrimps and was suggested to be an indicator of reproductive performance for selective breeding .As a result, Lim-nodrilus hoffmeisteri is a suitable substitute of Nereis succinea based on its effect on the reproductive traits and VTG mRNA expression level in parent shrimps of Litopenaeus vannamei.关键词
卵黄蛋白原/基因表达/促熟/南美白对虾/水丝蚓Key words
Vitellogenin/gene expression/ripening/Litopenaeus vannamei/Limnodrilus hoffmeisteri分类
生物科学引用本文复制引用
彭金霞,胡睿宇,陈晓汉,蒋小珍,韦嫔媛,李咏梅,陈秀荔,蒋伟明..基于卵黄蛋白原基因表达的水丝蚓促熟南美白对虾研究[J].水生态学杂志,2014,(2):74-79,6.基金项目
广西自然科学基金项目（2011GXNSFD018020）；广西科学研究与技术开发计划项目（桂科合1140010-17）；现代农业产业技术体系建设专项（CARS-47）。 （）
