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敌百虫胁迫对克氏原螯虾免疫学相关指标的影响

钟君伟 朱永安 孟庆磊 张龙岗 安丽

水生态学杂志Issue(2):95-100,6.
水生态学杂志Issue(2):95-100,6.

敌百虫胁迫对克氏原螯虾免疫学相关指标的影响

Effects of the Stress of Trichlorphon on Some Immune Parameters of Procambarus clarkii

钟君伟 1朱永安 1孟庆磊 1张龙岗 1安丽1

作者信息

  • 1. 山东省淡水渔业研究院,山东省淡水水产遗传育种重点实验室,济南 250013
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摘要

Abstract

To investigate the influence of trichlorphon stress on the immune parameters of Procambarus clarkii, Pro-cambarus clarkii for the experiment were reared in the test solution of 0 mg/L, 0.132 mg/L,0.264 mg/L and 0.528 mg/L trichlorphon for 12 days, respectively.The activities of four nonspecific immune parameters (T-SOD, CAT,ACP and AKP) in hepatopancreas and two in muscle (Na+,K +-ATPase and TChE) were measured.The re-sults showed that the effects of trichlorphon on the immune parameters were different .The activity of T-SOD in-creased significantly after exposure to 0.132 mg/L trichlorphon for 4 days and 0.528 mg/L trichlorphon for 1 day. The activity of CAT was significantly higher compared with the control group at the concentration of 0.132 mg/L and 0.264 mg/L after 2 days.The activities of ACP and AKP were always lower than those of the control group during the period of 12 days in all groups , and ACP activity decreased continuously from 1 d to 4 d.The activity of TChE in muscle increased at the concentration of 0.132 mg/L and 0.264 mg/L trichlorphon after 1 day and 4 days, respectively. No difference in Na +, K+-ATPase activity was observed except the test groups of 0 .528 mg/L trichlorphon with Na +, K+-ATPase activity significantly higher than that of the control group within 2 days(P<0.05).The study suggested that the immune parameters of Procambarus clarkia in each test group were significantly lower than those of the control groups after 12 days, and they couldn′t restore to the normal level.

关键词

克氏原螯虾/敌百虫/胁迫/免疫因子

Key words

Procambarus clarkii/trichlorphon/stress/immune parameter

分类

生物科学

引用本文复制引用

钟君伟,朱永安,孟庆磊,张龙岗,安丽..敌百虫胁迫对克氏原螯虾免疫学相关指标的影响[J].水生态学杂志,2014,(2):95-100,6.

基金项目

山东省科技发展计划项目(2010GCG20901);山东省海洋与渔业厅科技计划项目。 ()

水生态学杂志

OA北大核心CSCDCSTPCD

1674-3075

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