生物加工过程Issue(1)：18-22,5.DOI:10.3969/j.issn.1672-3678.2014.01.004
皮状丝孢酵母同步利用葡萄糖／木糖的糖转运动力学
Kinetics of sugar transport by Trichosporon cutaneum using simultaneous utilization of glucose and xylose
摘要
Abstract
The yeast Trichosporon cutaneum can utilize glucose and xylose simultaneously for microbial lipid production. The sugar transport kinetics was studied by using 2-deoxyglucose ( 2-DOG ) as a surrogate substrate.It was found that kinetics data of 2-DOG transport fitted well to Michaelis-Menten plot and that the apparent Km of 0.19 mmol/L and Vmax of 14.1 nmol/( min·mg) were obtained.Both glucose and xylose inhibited competitively the transport of 2-DOG, indicating that these compounds shared the same transporter.The apparent inhibition constant Ki of glucose was much lower than that of xylose, suggesting a higher affinity of the transporter to glucose than xylose.The fact that large amount of xylose was uptaken together with 2-DOG further supported the co-transport of glucose and xylose.As 2-DOG transport was sensitive to metabolic inhibitors and pH,an H+/sugar-symport system was suggested as the main sugar transporter.关键词
同步利用/糖转运/同向运输/2-脱氧葡萄糖/皮状丝孢酵母Key words
simultaneous utilization/sugar transport/symport system/2-deoxyglucose/Trichosporon cutaneum分类
化学化工引用本文复制引用
胡翠敏,王倩,龚志伟,杨晓兵,靳国杰,沈宏伟,赵宗保..皮状丝孢酵母同步利用葡萄糖／木糖的糖转运动力学[J].生物加工过程,2014,(1):18-22,5.基金项目
国家重点基础研究发展计划（973计划） （）
