生物加工过程Issue(2)：1-5,5.DOI:10.3969/j.issn.1672-3678.2014.02.001
沙柳原料高浓度底物酶解发酵乙醇工艺
Process of high-solid loadings on enzymatic hydrolysis and ethanol fermentation of Salix biomass
摘要
Abstract
In order to improve the economic benefit of the Salix biorefinery process,this paper examined the Salix pretreatment by steam explosion,ultrafine grinding with dilute acid,ultrafine grinding with dilute alkali,followed by high solid concentration of enzymatic hydrolysis,and ethanol fermentation by yeast.The results showed that steam explosion is the best pretreatment method for Salix enzymatic hydrolysis.The substrate concentration could reach 30% by feeding enzymatic method.The enzymatic hydrolyzate of total sugar concentration was 132 g/L and the glucose was 105 g/L.When using the ultrafine grinding and dilute acid pretreated material,the substrate concentration could reach 22%, the enzymatic hydrolyzate of total sugar concentration was 123 g/L and glucose concentration was 73 g/L.When using the ultrafine grinding and dilute alkali pretreated material,the substrate concentration could reach 22%,the enzymatic hydrolyzate of total sugar concentration was 133 g/L and glucose concentration was 77 g/L.The enzymatic hydrolyzate of three pretreatment methods for Salix could be fermented to ethanol by yeast and the hydrolyzate of steam explosion pretreated materials could reach 47 g/L ethanol by fermentation.关键词
乙醇/发酵/高浓酶解/预处理/沙柳Key words
ethanol/fermentation/high-solid loading hydrolysis/pretreatment/Salix分类
农业科技引用本文复制引用
林增祥,李荣秀,刘莉,沈兆兵,史吉平..沙柳原料高浓度底物酶解发酵乙醇工艺[J].生物加工过程,2014,(2):1-5,5.基金项目
上海市科委项目（10dz1210400）；上海浦东博士后基金 （）
