生物加工过程Issue(2)：6-11,6.DOI:10.3969/j.issn.1672-3678.2014.02.002
离子液体修饰的SBA 16材料在猪胰脂肪酶固定化中的应用
Immobilization of porcine pancreatic lipase on ionic liquid modified mesoporous silica SBA-16
摘要
Abstract
Ionic liquid was synthesized and grafted on the surface hydroxyl of mesoporous silica SBA-16 (ILSBA 16).Porcine pancreatic lipase(PPL) was immobilization on the ionic liquid modified SBA-16 material by physical adsorption ( PPL-ILSBA-16 ) .The enzymatic properties, including optimum temperature, pH and reusability of PPL-ILSBA-16, were investigated in the triacetin hydrolysis reaction.Compared with parent SBA-16 immobilized lipase( PPL-SBA-16) ,the enzymatic properties were dramatically improved, especially the optimized activity increased from 261 to 456 U/g PPL after modification.Characterizations with N2 adsorption, FT-IR and TG revealed that introduction of IL influenced the catalytic behaviors significantly by changing structure and surface properties of the carriers.关键词
固定化/离子液体/介孔材料/脂肪酶/修饰Key words
immobilization/ionic liquid/mesoporous material/lipase/modification分类
化学化工引用本文复制引用
邹彬,初旭明,张洋,胡燚..离子液体修饰的SBA 16材料在猪胰脂肪酶固定化中的应用[J].生物加工过程,2014,(2):6-11,6.基金项目
国家重点基础研究发展计划（973计划）（2011CB710800）；国家高技术研究发展计划（863计划）（2011AA02A209）；国家自然科学基金青年基金（20906049）；国家自然科学基金重点项目（20936002）；南京工业大学自然科学基金；南京工业大学材料化学工程国家重点实验室开放课题 （）
