生物加工过程Issue(2)：17-23,7.DOI:10.3969/j.issn.1672-3678.2014.02.004
黑曲霉产菊粉酶菌株的选育及培养基优化
Breeding of Aspergillus niger strain for producing inulinase and optimization of culture medium
摘要
Abstract
In order to acquire a high-yield Aspergillus niger strain for producing inulinase,the original strain Aspergillus niger YH-1 was treated with N-methyl-N′-nitro-soguanidine ( NTG ) and domesticated by combining high temperature and Jerusalem artichoke powder, a inulinase production strain YH-3 was selected.The culture medium of YH-3 was optimized by response surface methodology.The results showed that the optimum culture medium components were determined:Jerusalem artichoke flour 25.2 g/L, soybean cake powder 40 g/L, sucrose ester 4.9 g/L and NaCl 5.5 g/L.Under the optimum conditions, the yield of inulinase was increased from 60.9 U/mL to 165.0 U/mL, increased 1.7 times compared with the starting strain.The research proved that sucrose ester was an effective accelerator for Aspergillus niger YH-3 producting inulinase.关键词
黑曲霉/驯化/菊粉酶/诱变/响应面Key words
Aspergillus niger/domestication/inulinase/mutation/response surface methodology分类
生物科学引用本文复制引用
徐艳新,张伟国,葛向阳..黑曲霉产菊粉酶菌株的选育及培养基优化[J].生物加工过程,2014,(2):17-23,7.基金项目
中国博士后科学基金 （）
