生物加工过程Issue(2)：28-32,5.DOI:10.3969/j.issn.1672-3678.2014.02.006
代谢木糖重组谷氨酸棒杆菌的构建
Construction of xylose-utilizing recombinant Corynebacterium glutamicum strain
摘要
Abstract
In order to construct a strain of Corynebacterium glutamicum by using the xylose to produce organic acids,the xylA gene from Escherichia coli K-12,encoding xylose isomerase,was integrated in the expression vector of pXMJ19.The gene was expressed in the Corynebacterium glutamicum ATCC13032Δldh strain.The recombinant strain was capable of growth on xylose as a sole carbon source,the xylose consumption rate was 0.54 g/( L·h ).The xylose isomerase activity reached 0.54 U/mL.In medium containing glucose and xylose, the recombinant strain consumed glucose first, after the glucose was consumped entirely, the effective utilization of xylose was started.The yield of succinate was ( 0.62 ± 0.003) g/g during the two-stage fermentation on xylose as a sole carbon source.关键词
谷氨酸棒杆菌/琥珀酸/木糖异构酶基因/木糖Key words
Corynebacterium glutamicum/succinic acid/xylA/xylose分类
生物科学引用本文复制引用
张恒丽,蔡恒,汪晨,张凯,周志惠..代谢木糖重组谷氨酸棒杆菌的构建[J].生物加工过程,2014,(2):28-32,5.基金项目
国家重点基础研究发展计划（973计划） （）
