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微生物油脂中花生四烯酸含量的准确快速测定

颜佳铖 纪晓俊 聂志奎 邓中涛 任路静 黄和

生物加工过程Issue(2):66-69,4.
生物加工过程Issue(2):66-69,4.DOI:10.3969/j.issn.1672-3678.2014.02.013

微生物油脂中花生四烯酸含量的准确快速测定

Fast determination of arachidonic acid in microbial oils

颜佳铖 1纪晓俊 1聂志奎 1邓中涛 1任路静 1黄和1

作者信息

  • 1. 南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室,南京 210009
  • 折叠

摘要

Abstract

Gas chromatography was performed for the determination of arachidonic acid ( ARA) produced by micro-organisms.Capillary column DB-23 was used with FID and suitable carrier gas for the detection.The result indicated that the method could separate fatty acids well in 20 min on good precision and recovery.The recovery rate of ARA was 90.146% to 100.634%,and the relative standard deviation was 4.175%.Thus,the method was effective for the detection of ARA produced by micro-organisms.

关键词

ARA/气相色谱分析/微生物油脂

Key words

arachidonic acid/gas chromatography/microbial oils

分类

生物科学

引用本文复制引用

颜佳铖,纪晓俊,聂志奎,邓中涛,任路静,黄和..微生物油脂中花生四烯酸含量的准确快速测定[J].生物加工过程,2014,(2):66-69,4.

基金项目

国家高技术研究发展计划(863计划)(2014AA021703);国家自然科学基金(21376002);江苏省自然科学基金(BK20131405);江苏高校优势学科建设工程 ()

生物加工过程

OACSTPCD

1672-3678

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