生物技术通报Issue(4)：64-70,7.
青杄生长素抑制蛋白基因PwARP-1的克隆及表达分析
The Cloning and Expression Analysis of PwARP-1 in Picea wilsonii
摘要
Abstract
An auxin repressed protein gene PwARP-1 is cloned from Picea wilsonii. The full length cDNA of PwARP-1 is obtained by RACE-PCR assays based on the cDNA library of Picea wilsonii and EST fragment of PwARP-1. Bioinformatic tools are applied for the prediction of PwARP-1. PwARP-1 encodes a protein with 155 amino acids. The molecular weight is 17.1 kD and theoretical pI is 10.07 and the protein is rich in random coil. The tissue-specific expressions and the response to phytohormones of PwARP-1 were investigated by RT-qPCR assays. The tissue-specific expression assays suggested that the expression level in pollen was the highest among all tissues and the expression level in seed was the lowest. During seed germination, the expression of PwARP-1 was increased, peaked at the 10th day and then decreased. Furthermore, the expression of PwARP-1 was significantly inhibited by Auxin and Gibberellin. However, the expression level of PwARP-1 is up-regulated by Brassinolide and drought stress treatment. The expression level of PwARP-1 declined and then up-regulated under salt stress, methyl jasmonate, and abscisic acid treatments. These results indicate that PwARP-1 has a potential function in seed germination and responding to stresses and phytohormone treatments.关键词
青杄/生长素抑制蛋白/表达分析/植物激素Key words
Picea wilsonii/Auxin-repressed protein/Expression analysis/Phytohormone引用本文复制引用
孙帆,罗朝兵,周燕妮,张凌云..青杄生长素抑制蛋白基因PwARP-1的克隆及表达分析[J].生物技术通报,2014,(4):64-70,7.基金项目
国家自然科学基金面上项目（31270663），国家转基因生物新品种培育科技重大专项 （）
