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香蕉MaBTB基因的表达分析及亚细胞定位

柴文龙 金志强 贾彩红 刘菊华 苗红霞 张建斌 徐碧玉

生物技术通报Issue(4):71-76,6.
生物技术通报Issue(4):71-76,6.

香蕉MaBTB基因的表达分析及亚细胞定位

Expression Analysis and Subcellular Localization of the MaBTB Gene in Banana

柴文龙 1金志强 2贾彩红 3刘菊华 3苗红霞 3张建斌 3徐碧玉3

作者信息

  • 1. 海南大学农学院,海口 570228
  • 2. 中国热带农业科学院海口实验站 海南省香蕉遗传改良重点实验室,海口 570102
  • 3. 中国热带农业科学院热带生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室,海口571101
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摘要

Abstract

Real-time quantitative PCR of postharvest banana revealed that MaBTB exhibited differential expression patterns that are associated with ethylene biosynthesis. In naturally ripened bananas, expression of MaBTB was in accordance with ethylene biosynthesis. In contrast, for 1-methylcyclopropene-treated bananas, MaBTB expression levels remained constant. Following treatment with ethylene, MaBTB expression in banana fruit significantly increased with ethylene biosynthesis and peaked 3 d after harvest, which was 11 d earlier than that for naturally ripened banana fruits. These results suggested that MaBTB expression was induced by ethylene for regulation of postharvest banana ripening. Finally, subcellular localization assays showed that the MaBTB protein localizes to the nucleus.

关键词

香蕉/MaBTB基因/表达分析/亚细胞定位

Key words

Banana(Musa acuminata L. AAA group)/MaBTB gene/Expression analysis/Subcelluar localization

引用本文复制引用

柴文龙,金志强,贾彩红,刘菊华,苗红霞,张建斌,徐碧玉..香蕉MaBTB基因的表达分析及亚细胞定位[J].生物技术通报,2014,(4):71-76,6.

基金项目

中央级公益性科研院所基本科研业务专项(ITBB110216),海南省重大科技项目子课题 ()

生物技术通报

OA北大核心CSCDCSTPCD

1002-5464

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