生物技术通报Issue(4):71-76,6.
香蕉MaBTB基因的表达分析及亚细胞定位
Expression Analysis and Subcellular Localization of the MaBTB Gene in Banana
摘要
Abstract
Real-time quantitative PCR of postharvest banana revealed that MaBTB exhibited differential expression patterns that are associated with ethylene biosynthesis. In naturally ripened bananas, expression of MaBTB was in accordance with ethylene biosynthesis. In contrast, for 1-methylcyclopropene-treated bananas, MaBTB expression levels remained constant. Following treatment with ethylene, MaBTB expression in banana fruit significantly increased with ethylene biosynthesis and peaked 3 d after harvest, which was 11 d earlier than that for naturally ripened banana fruits. These results suggested that MaBTB expression was induced by ethylene for regulation of postharvest banana ripening. Finally, subcellular localization assays showed that the MaBTB protein localizes to the nucleus.关键词
香蕉/MaBTB基因/表达分析/亚细胞定位Key words
Banana(Musa acuminata L. AAA group)/MaBTB gene/Expression analysis/Subcelluar localization引用本文复制引用
柴文龙,金志强,贾彩红,刘菊华,苗红霞,张建斌,徐碧玉..香蕉MaBTB基因的表达分析及亚细胞定位[J].生物技术通报,2014,(4):71-76,6.基金项目
中央级公益性科研院所基本科研业务专项(ITBB110216),海南省重大科技项目子课题 ()