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一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定

王菲 刘抗 范家同 成守亮 王子元

生物技术通报Issue(4):127-131,5.
生物技术通报Issue(4):127-131,5.

一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定

Isolation and Identification of a Halotolerant-Vibrio sp. K1-L

王菲 1刘抗 1范家同 1成守亮 1王子元1

作者信息

  • 1. 江苏师范大学生命科学学院,徐州 221116
  • 折叠

摘要

Abstract

Strain K1-L, isolated from Yuncheng Salt Lake, Shanxi Province, could swim on the 2%-10%MGM medium. The strain K1-L was Gram-negative with polar flagellum and was 0.4μm ×(1-2)μm under the scanning electron microscope. Strain K1-L appeared grey, rounded colony. Its optimum salt concentration, temperature and pH ranges were 2%-6%, 30-40℃and 6.0-8.0. The GC content is 47.06%. After comparing the traditional characteristics of morphology, physiological biochemistry and 16S rRNA sequence analysis, we discovered that the strain K1-L was highly homologous to the Vibrio diabolicus HE800T and Vibrio rotiferianus LMG 21460T. The maximum similarity was 99%. It was asserted that K1-L was belonged to the genus Vibrio, we proposed the name Vibrio sp. K1-L for this strain.

关键词

运城盐湖/弧菌属/耐盐菌/系统发育分析

Key words

Yuncheng salt lake/Vibrio/Halotolerant/Phylogenetic analysis

引用本文复制引用

王菲,刘抗,范家同,成守亮,王子元..一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定[J].生物技术通报,2014,(4):127-131,5.

基金项目

国家自然科学基金项目(30571071),江苏师范大学王子元教授科研启动基金(KY2009101),江苏高校优势学科建设工程项目,江苏省教育厅优秀创新团队项目 ()

生物技术通报

OA北大核心CSCDCSTPCD

1002-5464

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