生物技术通报Issue(4)：132-138,7.
短小芽孢杆菌脂肪酶基因的克隆、表达及酶学性质研究
Gene Cloning,Expression and Characterization of the Lipase from Bacillus pumilus S6
摘要
Abstract
A lipase-producing strain was screened from the soil samples. It was identified as Bacillus pumilus by the 16S rRNA method. According to the reported lipase genes from Bacillus, the primers were derived and the full-length gene was cloned, named“lipS6”, which had the size of 648 bp, encoding 215 amino acids.“lipS6”held 96%homology with the reported lipase gene B26. Recombinant plasmid pET32a-lipS6 was construced and expressed in E. coli.“lipS6”could be expressed in soluble form, and the lipase activity reached 2 000 U/L. The optimum temperature of recombinant lipase LipS6 was 30℃, and the optimum pH was 9. Low concentrations of Ca2+and Mn2+could activate the activity of lipase LipS6. The harmful effect of hydrophobic organic solvent on the lipase LipS6 was little. LipS6 could catalyze the esterification reaction in the n-hexane system, with the myristic acid and n-butanol as the optimum substrates. These properties show that LipS6 can be used in the fields of pulp and paper, leather, textiles and bioenergy.关键词
脂肪酶/短小芽孢杆菌/基因克隆/重组表达/性质Key words
Lipase Bacillus pumilus/Gene cloning/Recombinant expression/Characterization引用本文复制引用
苏二正,吴向萍,高蓓,魏东芝..短小芽孢杆菌脂肪酶基因的克隆、表达及酶学性质研究[J].生物技术通报,2014,(4):132-138,7.基金项目
国家高技术研究发展计划（“863”计划）（2012AA020403），国家自然科学基金项目（31201296/C200101），生物反应器工程国家重点实验室开放课题，南京林业大学引进高层次人才科研基金项目，江苏高校优势学科建设工程资助项目 （）
