生物技术通报Issue(4)：152-158,7.
大伏革菌产纤维素酶条件优化及高效菌株筛选
Optimization of Submerged Culture Requirements for the Cellulase Activity by Phlebiopsis gigantea and Screening the Most Effective Strain
摘要
Abstract
The activity of extracellular cellulase could be used for screening the effective biocontrol strain. We could acquire the most effective Phlebiopsis gigantea isolate associated with biocontrol conifer root and butt rots by determining cellulase activity of them. Based on single factor and orthogonal experiment, the optimum medium compositions for the highest cellulase activity were(g/L):glucose 30.00, peptone 5.00, KH2PO4 3.00, MgSO4·7H2O 1.50, the optimal liquid medium volume was 120 mL/250 mL, the optimal inoculum volume was 5%(V/V), initial pH4.0. By determining cellulase activity of P. gigantea isolates, we concluded that P. gigantea 08077 had the highest cellulase activity and 13025 was the lowest. P. gigantea 08077 is better to control the Chinese Heterobasidion parviporum than Rotstop-F that had been registered in Finland.关键词
大伏革菌/液体培养/优化/纤维素酶活力/正交试验Key words
Phlebiopsis gigantea/Submerged culture/Optimization/Cellulase activity/Orthogonal array design引用本文复制引用
李杏春,何双辉,戴玉成..大伏革菌产纤维素酶条件优化及高效菌株筛选[J].生物技术通报,2014,(4):152-158,7.基金项目
引进国际先进林业科学技术项目 （）
