生物技术通报Issue(4)：159-163,5.
产耐酸性α-淀粉酶菌株的分离、鉴定、酶学特性研究及发酵培养基的优化
Screening,Identification and Characterization of an Acidα-amylase Producing Strain and Optimization of Its Fermentable Condition
摘要
Abstract
Multiple new acidα-amylase producing strains were isolated from soil which is rich in starch. A strain which has the highest enzyme activity was identified as Bacillus subtilis A-7 through analysis of 16S rDNA gene. The optimum temperature and pH was 60℃and 4.5. The single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium. The composition of the best medium was 2%soluble starch, 2%peptone, 0.07%CaCl2, 0.8%Na2HPO4. Under this optimal condition, the activity of acidα-amylase was 221 U/mL and 2.3-fold more than that of no optimization.关键词
耐酸性α-淀粉酶/16S rDNA鉴定/枯草芽孢杆菌/最适温度/最适pH/正交试验Key words
Acidα-amylase/16S rDNA/Bacillus subtilis/Optimum temperature/Optimum pH/Orthogonal experiment引用本文复制引用
王建玲,陈志鑫,刘逸寒,路福平..产耐酸性α-淀粉酶菌株的分离、鉴定、酶学特性研究及发酵培养基的优化[J].生物技术通报,2014,(4):159-163,5.基金项目
天津科技大学实验室开放基金项目（1204A211），国家自然科学基金资助项目（31101219），国家高技术研究发展计划（“863”计划）项目 （）
