生物技术通报Issue(4)：169-175,7.
自养条件下高氯酸盐降解细菌群落研究
The Study of the Structure of Perchlorate(ClO4-)-degrading Bacterial Communities Under Autotrophic Conditions
摘要
Abstract
For the purpose of further investigating the biological degradation under an autotrophic condition and well understanding the microbial community structures in a complex environment, hydrogen was used as an electron donor to completely reduce perchlorate(ClO4-)in this study. The composition of microbial communities after degradation was analyzed via the construction of a cloning library by using the High-Throughput Sequencing method(HiSeq 2000). 71 days were needed to completely degrade 10 mg/L ClO4-. Microbial phylogenic analysis of HD(hydrogen degradation)after degradation indicated that the relative abundance of total bacteria in the HD was 84.96%whereas the relative abundance of Proteobacteria was 68.11%, whose percentage accounting for the total bacteria reached to 80.16%. The relative abundance of Dechloromonas which is representative in PRB was 2.7%in the HD. Simultaneously, the relative abundance of Azospira was 3.1%. KEGG was used to analyze the function of bacteria in HD. The relative abundance of genes which engaged in carbohydrate metabolism was 4.75%, and the genes included in energy metabolism was 3.35%, whereas the genes participated in nitrogen cycle was 0.72%, and the genes involving chloride transformation was 0.83%. It was demonstrated that degradation of ClO4-in a complicated condition was achieved by various kinds of microbes rather than a single one. Adding hydrogen as an electron donor to change microbial community played a role in the purification or selection process in the system, which allowed the complex systems to have the specific capacity to remove given contaminates.关键词
高氯酸根离子(ClO4-)/电子供体/氢气/细菌群落结构/高通量测序Key words
Perchlorate(ClO4-)/Electron donor/Hydrogen/The composition of microbial communities/High-Throughput Sequencing引用本文复制引用
谢宇轩,关翔宇,于丽莎,刘菲..自养条件下高氯酸盐降解细菌群落研究[J].生物技术通报,2014,(4):169-175,7.基金项目
国家自然科学基金项目（41272268），中国地质调查局项目（1212011121171），中央高校基本科研业务费专项资金 （）
