实用肝脏病杂志Issue(5)：401-403,404,4.DOI:10.3969/j.issn.1672-5069.2013.05.006
GPC-3特异性短发夹型RNA干预基因转录对肝癌细胞增殖与凋亡的影响*
Effects of glypican-3 gene k nockdown by shRNA on proliferation and apoptosis of HepG2 cells
摘要
Abstract
Objective To investigate the effects of glypican-3 (GPC-3)gene knockdown by specific short hairpin RNA(shRNA)on proliferation and apoptosis of HepG2 cells. Methods Four pairs of GPC-3 shRNA were designed,synthesized and screened according to the inhibiting efficiency and plasmids containing specific GPC-3 shRNA were constructed;HepG2 cells were transfected with constructed plasmid containing GPC-3 shRNA with the highest inhibiting rate,and cell proliferation,cell cycle transition,and apoptosis were determined after GPC-3 knockdown. Results Four plasmids containing specific GPC-3 shRNA were successfully constructed,and one of which had the highest inhibiting rate by reaching to 89.3%;The proliferation rates of the HepG2 cells transfected with the efficient GPC-3 shRNA decreased to 33.2%,56.0% and 71.1%,respectively,as compared to the corre-sponding controls(P<0.05) 24h,48h and 72 hours after transfection;The cell cycles were arrested at G1 phase af-ter GPC-3 knockdown,while the apoptosis rate of transfected HepG2 cells increased to 65.6%,significantly higher than that in control (5%,P<0.05). Conclusions Specific GPC-3 shRNA can effectively knockdown GPC-3 gene transcription in HepG2 cells,resulting in cell proliferation inhibition,cell cycle arrest and increased apoptosis;GPC-3 might be a potential target gene for hepatocellular carcinoma therapy.关键词
HepG2细胞/磷脂酰肌醇蛋白多糖-3/短发夹型RNA/基因沉默Key words
HepG2 cells/Glypican-3/Short hairpin RNA/Gene silencing引用本文复制引用
邰伯军,姚敏,蔚丹丹,陈洁,时运,顾星,张洁,邱历伟,姚登福..GPC-3特异性短发夹型RNA干预基因转录对肝癌细胞增殖与凋亡的影响*[J].实用肝脏病杂志,2013,(5):401-403,404,4.基金项目
国家国际科技合作专项(2011ZR0003)；国家和江苏省博士后基金资助项目 （2011ZR0003）
