摘要
Abstract
Objective To investigate the changes in peripheral T lymphocyte subsets and natural killer (NK)cells in patients underwent radiofrequency ablation(RFA)for primary liver cancer. Methods 23 patients with primary liver cancer were recruited and received RFA treatment. The changes in the proportion of CD3+Tcell,CD4+Tcell,CTL cell,NK cells,and CD4+/CD8+ ratio in peripheral blood in 23 healthy controls and in 23 patients with primary liver cancer before and after RFA treatment were determined by flow cytometry. Results The proportion of CD3+Tcell,CD4+Tcell,CTL cell,NK cell and the CD4+/CD8+ ratio in patients with primary liver cancer were 61.9±13.8%,33.3±5.0%,10.3±4.9%,4.4±1.3% and 1.4±0.6,respectively,all of which were significantly lower than those in healthy controls (75.7±12.3%,41.1±10.1%,19.7±5.8%,16.7±8.2%,1.7±0.6,P<0.05);In contrast,the proportion of CD8+T cell (25.5±5.1%)in patients was higher than that in healthy controls (21.7±6.7%,P<0.05);In patients with primary liver cancer,the proportion of CD3+T cell(72.1±9.8%,73.3±7.3%),CD4+T cell(39.6±10.9%,39.0±10.0%), CTL cell(14.8±9.6%,12.3±7.4%)and NK cell(11.1±4.9%,12.8±8.1%)were increased,while the proportion of CD8+T cell(22.6±5.8%,22.0±9.6%)was reduced,and the CD4+/CD8+ ratio(1.7±0.8,1.9±0.4)was decreased(P<0.05)one or two weeks after RFA as compared to that before RFA. Conclusion Most of the patients with primary liver can-cer are in immunosuppressed status,and RFA therapy can adjust the balance in T cell subsets and increase NK cell counts.关键词
原发性肝癌/射频消融术/T淋巴细胞亚群/自然杀伤细胞Key words
Primary liver cancer/Radiofrequency ablation/T lymphocyte subsets/Natural killer cell
