实用肝脏病杂志Issue(6)：523-525,3.DOI:10.3969/j.issn.1672-5069.2013.06.015
携TIMP-1基因RNAi慢病毒载体感染大鼠HSC-T6细胞抑制目的基因表达
Lentiviral vector containing TIMP-1 gene-specific small interfering RNA inhibited target gene expres-sion in HSC-T6 cells
摘要
Abstract
Objective To explore the inhibiting effect of constructed lentiviral vector containing tissue in-hibitor of metalloproteinase-1 (TIMP-1)gene-specific small interfering RNA on target gene expression in hepatic stellate cells-T6 (HSC-T6). Methods HSC-T6 cells were infected with lentiviral virus plasmid for 6 to 8 days. Real-time PCR was used to detect the infection efficiency at 6 days;Western blot was performed to detect the protein levels of TIMP-1 at 6 and 8 days. Results TIMP-1 mRNA was reduced to 0.668 time that of the nor-mal control at 6 days[2-△△Ct was (0.668±0.046)],and this decrease was significantly higher than that in the nega-tive control [2-△△Ct was (1.001±0.041),(P<0.05)];Similarly,TIMP-1 protein was significantly reduced at 6 and 8 days after viral infection. Conclusion Constructed lentiviral vector containing TIMP-1 gene-specific small inter-fering RNA can effectively infect rat HSC-T6 cells and reduce the target gene expression.关键词
HSC-T6细胞/基质金属蛋白酶组织抑制因子-1/RNA干扰/慢病毒Key words
HSC-T6 cells/Tissue inhibitor of matrix metalloproteinase-1/RNA interference/Lentiviral vector引用本文复制引用
张素梅,郝春秋,康文臻,彭梅娟,贾战生,谢玉梅..携TIMP-1基因RNAi慢病毒载体感染大鼠HSC-T6细胞抑制目的基因表达[J].实用肝脏病杂志,2013,(6):523-525,3.基金项目
国家自然科学基金项目（81170389）；陕西省自然科学基础研究计划项目 （）
