实用肝脏病杂志Issue(6)：526-529,4.DOI:10.3969/j.issn.1672-5069.2013.06.016
实验性肝纤维化大鼠肝组织CTGF基因DNA甲基化的变化
DNA methylation of CTGF gene in liver tissues of rats with CCl4-induded fibrosis
摘要
Abstract
Objective To investigate the change in DNA methylation of connective tissue growth factor(CT-GF) promoter and its effect on CTGF gene expression in experimental liver fibrosis in rats. Methods A rat model of liver fibrosis was established by subcutaneous injection of carbon tetrachloride for 6 weeks. The patho-logical changes in fibrotic livers were examed by HE and Sirius red staining; The levels of CTGF mRNA and protein expression in liver tissues were evaluated by reverse transcription polymerase chain reaction (RT-PCR)and Western blot,respectively;DNA methylation of CTGF gene was determined by sequencing after bisulfite modifica-tion. Results Liver fibrosis of grade III was observed after carbon tetrachloride injection at the end of 6 weeks. In liver tissues from model rats,the CTGF mRNA and protein were(1.581±0.276) and(0.875±0.102),respectively, which were significantly higher than those in the control rats(0.415±0.041)and(0.115±0.083),respectively,(P<0.01);Importantly,in liver tissues from models rats,the DNA methylation rate of CTGF gene promoter was(1.8±1.8)%, significantly lower than that in liver tissue from control rats (11.6±3.8)%,(P<0.01). Conclusion CTGF gene is upregulated in liver fibrosis,and this might be resulted from decreased DNA methylation in CTGF gene promoter.关键词
肝纤维化/结缔组织生长因子/DNA甲基化/大鼠Key words
Liver fibrosis/Connective tissue growth factor/DNA methylation/Rats引用本文复制引用
李光明,王军,潘勤,葛文松,范建高..实验性肝纤维化大鼠肝组织CTGF基因DNA甲基化的变化[J].实用肝脏病杂志,2013,(6):526-529,4.基金项目
国家自然科学基金（81070344&8107322）；中国肝炎基金会王宝恩肝纤维化研究基金（20090007）；上海市科委“创新行动计划”（09140903500）；国家科技部973课题 （）
