实用肝脏病杂志Issue(1)：30-33,4.DOI:10.3969/j.issn.1672-5069.2014.01.009
他莫昔芬体外促进HepG2细胞脂肪变性观察
Tamoxifen promotes lipid accumulation in HepG2 cells in vitro
摘要
Abstract
Objective To investigate the effect of tamoxifen(TAM) on steatosis in HepG2 cells in vitro and on the expression of key regulators involved in lipid metabolism in the cells. Methods A cell model of steatosis was induced in HepG2 cells in vitro with oleic acid (OA) at 50 μmol/L;HepG2 cells were then subjected to dif-ferent concentrations of TAM (5 to 20 μmol/L) at the presence of OA for 72 h;Intracellular lipid accumulation was assessed by oil red O staining and measurement of triglyceride;The expression of sterol regulatory element-binding protein-1c(SREBP-1c),fatty acid synthase(FAS),steroyl-CoA desaturase(SCD),carnitine palmitoyltrans-ferase 1(CPT1)and mitochondrial trifunctional protein(MTP)was determined by Western blot;Cell viability was de-tected by cell counting Kit-8 assay. Results After incubation for 72 h,the intracellular triglyceride in control group was (16.53±0.17) mg/100 mg protein,similar to that of cells treated with 5μmol/L TAM,however,the intra-cellular triglyceride was increased by 31%[(21.57±0.16) mg/100 mg protein] and 44%[(23.82±0.44) mg/100 mg protein] in cells treated with 10 μmol/L and 20 μmol/L of TAM,respectively(P<0.05);TAM treatment(5 to 10 μmol/L) significantly increased the expression of SREBP-1c,FAS,SCD and MTP without affecting the expression of carni-tine palmitoyltransferase 1 (CPT1) in HepG2 cells;TAM did not affect HepG2 viability. Conclusions TAM pro-motes OA-induced cell steatosis,probably by up-regulation of SREBP-1c,FAS and SCD,thus increases fatty acid synthesis in the cells.关键词
HepG2细胞/他莫昔芬/脂肪变/甘油三酯/脂肪酸合成Key words
HepG 2 cells/Tamoxifen/Steatosis/Triglyceride/Fatty acid synthesis引用本文复制引用
赵斐,谢萍,姜佳丽,张令强,安威,展玉涛..他莫昔芬体外促进HepG2细胞脂肪变性观察[J].实用肝脏病杂志,2014,(1):30-33,4.基金项目
肝脏保护与再生调节北京市重点实验室课题 （）
