实用肝脏病杂志Issue(2)：159-162,4.DOI:10.3969/j.issn.1672-5069.2014.02.012
人源化抗肝癌双链抗体纳米颗粒对荷人肝癌裸鼠放射免疫显像研究
Radioimmunoimaging of 99mTc labelled humanized single-chain Fv dimers and immunonanoparticles for hepatocellular carcinoma in nude mice
摘要
Abstract
Objective To investigate the radioimmunoimaging of 99mTc labelled humanized single-chain Fv dimmers(BDM3) and immunonanoparticles for hepatocellular carcinoma in nude mice bearing human hepatocellu-lar carcinoma,and to study their application in diagnosis and therapy of hepatocellular carcinoma. Methods The label rate of 99mTc-BDM3 and 99mTc-BDM3 nanoparticles was determined by isotopic analyzer. The labelled BDM3 and BDM3 nanoparticles were injected intraperitoneally into the nude mice bearing human hepatocellular carcino-ma. At intervals of 1 h,4 h and 8 h,the mice in each group were imaged on a SPECT,and then were sacrificed immediately to get various organs and tumors. Radioactivity ratio of tumor to non-tumor (T/NT) was measured. Results The label rates of 99mTc-BDM3 and 99mTc-BDM3 nanoparticle were 91% and 92%,respectively;The posi-tive display rates by the antibody and its nanoparticle were both 100%;The ratio of T/NT were (1.63±0.17) and (3.63±0.21) 1 h,(3.82±0.24) and (5.63±0.26) 4 h,(0.48±0.21) and (1.41±0.32) 8 h after injection of 99mTc-BDM3 and 99mTc-BDM3 nanoparticle,respectively. The differences between two groups were statistical sig-nificance;Favorable images were obtained after 4 h. The labelled BDM3 and BDM3 nanoparticles concentrated in carcinoma and did not in nontumor,that showed an evident targeting effect in tumor. As a result of a double tar-geting effect,the BDM3 nanoparticles group had more concentration in tumor,and increased the imaging effect. Conclusions The humanized single-chain Fv dimmers (BDM3) and immunonanoparticles for hepatocellular car-cinoma has high affinity to nude mice bearing human hepatocellular carcinoma xenografts. They could be target carrier for diagnosis and therapy of hepatocellular carcinoma.关键词
肝癌/99mTc 标记/免疫纳米颗粒/放射免疫显像Key words
Hepatocellular carcinoma/99mTc label/Immunonanoparticles/Radioimmunoimaging引用本文复制引用
别彩群,梁旭竞,汤绍辉,李萌,孙士敏,范红梅..人源化抗肝癌双链抗体纳米颗粒对荷人肝癌裸鼠放射免疫显像研究[J].实用肝脏病杂志,2014,(2):159-162,4.基金项目
深圳市科技计划资助项目 （）
