实用肝脏病杂志Issue(2)：180-183,4.DOI:10.3969/j.issn.1672-5069.2014.02.017
鸦胆子油注射液对 H22细胞增殖的抑制作用及其机制探讨
Inhibition of proliferation of hepatoma cell line H22 cells by brucea oil in vitro and in vivo
宋艳丽 1薛瑞 1吴倩 1张玉洁1
作者信息
- 1. 441021 湖北省襄阳市中心医院药学部
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effect and related mechanisms of Brucea oil on prolifer-ation of H22 hepatoma cell line. Methods H22 cells cultured in vitro were incubated with different concentra-tion of Breucea oil (10~160 μg/ml) and cell proliferation was determined by MTT assay;Mice bearing H22 tu-mors were treated with Breacea oil for 10 days,and then the mice were scarified and tumor inhibitory rate, spleen index and thymus index were calculated;MTT was performed to detect the proliferation of spleen cells from tumor-bearing mice;The serum TNF-α was measured by enzyme-linked immunosorbent assay and serum trans-forming growth factor-α (TGF-α) levels by radioimmunoassay. Result The cell proliferation was inhibited by Brucea oil to 34.1%~52.31% of that of in the control at concentrations of 10 to 160 μg/ml;At concentration of 25 and 50 mg·kg-1,the tumor mass in tumor-bearing mice were reduced to (1.095±0.301) g and (0.920±0.250) g,respectively,and serum TGF-α levels decreased to(11.172±0.639) pg/ml and (14.438±0.587) pg/ml,respective-ly,significantly lower than those in non-treated tumor-bearing mice[(1.867±0.554) g and (16.354±0.762) pg/ml, respectively];In contrast,serum TNF-α levels increased to(28.132±2.456) pg/ml and(26.521±3.267)pg/ml,respec-tively,significantly higher than that in non-treated tumor-bearing mice [(20.231±2.614) pg/ml,P<0.05];Brucea oil also promoted the proliferation of spleen cells,but had no significant effect on the spleen index and thymus index as compared to those in tumor-bearing mice. Conclusion Brucea oil inhibits the proliferation of H22 cells in vitro and in vivo,and the anti-tumor effect might be related to the regulation of spleen cells in tumor-bearing mice.关键词
H22 细胞/鸦胆子油/脾细胞/肿瘤坏死因子 -α/转化生长因子 -αKey words
H22 cell line/Brucea oil/Proliferation/Spleen cells/Tumor necrosis factor -α/Transforming growth factor-α引用本文复制引用
宋艳丽,薛瑞,吴倩,张玉洁..鸦胆子油注射液对 H22细胞增殖的抑制作用及其机制探讨[J].实用肝脏病杂志,2014,(2):180-183,4.
