实用医学杂志Issue(3)：361-364,4.DOI:10.3969/j.issn.1006-5725.2014.03.008
Rab25基因对乳腺癌裸鼠移植瘤生长的影响
Effects of Rab25 gene on the growth of implanted tumor of breast cancer in nude mice
摘要
Abstract
Objective To establish nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene , and to observe the effects of Rab25 gene on the growth of implanted tumor. Methods Nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene were constructed through up-regulating or silencing the expression of Rab25 gene in breast cancer cells. The size of implanted tumor was measured, the growth curve of tumor was portrayed, and the expression of Rab25 gene in tumor tissues was detected. Results The tumorigenic effect on nude mice was the strongest and the highest in Rab25 up-regulating group , as compared to those in Rab25 interference group , original cells group, and Rab25 empty plasmid group. The expression of Rab25 mRNA in interference group was significantly reduced. Conclusions Rab25 is a cancer-promoting gene , its high expression could directly enhance the proliferation of breast cancer cells. RNA interference technology had the potential to provide new ideas for tumor targeting and gene therapy for breast cancer.关键词
乳腺肿瘤/Rab25基因/裸鼠/移植瘤Key words
Breast neoplasms/Rab25/Nude mice/Implanted tumor引用本文复制引用
张小彬,陆云飞,沈桂鑫,吴非..Rab25基因对乳腺癌裸鼠移植瘤生长的影响[J].实用医学杂志,2014,(3):361-364,4.基金项目
广西医疗卫生重点课题 （）
