实用医学杂志Issue(4)：511-514,4.DOI:10.3969/j.issn.1006-5725.2014.04.004
坎地沙坦对人乳腺癌细胞株MCF-7生长抑制作用的研究
The Effect of Candesartan on human breast cancer cell line MCF-7
摘要
Abstract
Objective To investigate the proliferation inhibition of angiotensin Ⅱ 1 receptor (AT1R) antagonist candesartan on human breast cancer cell line MCF-7. Methods MCF-7 cells were tested in vitro for cytotoxicity of candesartan with MTT method. The effect of candesartan on cell cycle and apoptosis of MCF-7 cells were evaluated by flow cytometry. Western Blot was applied to test the expression level of wild-type p53 and Survivin protein. Results After candesartan treatment, a dose- and time-dependent decreased proliferation in MCF-7 cells was observed. Induction of G1 cell cycle arrest and increased apoptosis in MCF-7 cells were also observed.Western blot assay showed that candesartan up-regulated wild-type P53 expression and down-regulated the Survivin expression in MCF-7 cells. Conclusions This study suggests that candesartan could inhibit the proliferation , induce G1 cell cycle arrest and apoptosis of MCF-7cellsby regulating the protein expression level of gene p53 and Survivin.关键词
乳腺肿瘤/坎地沙坦/细胞周期阻滞/凋亡/p53/SurvivinKey words
Breast carcinoma/Candesartan/Cell cycle arrest/Apoptosis/p53/Survivin引用本文复制引用
林淑慧,谢国柱,张静芳,蔡小慧,姚奇伟,孙权权,王玮,袁亚维..坎地沙坦对人乳腺癌细胞株MCF-7生长抑制作用的研究[J].实用医学杂志,2014,(4):511-514,4.基金项目
国家自然科学基金（编号81272508）；国家自然科学基金（编号81302326）；广东省自然科学基金（编号S2011040003465）；高等学校博士学科点专项科研基金 （）
