实用医学杂志Issue(4)：514-517,4.DOI:10.3969/j.issn.1006-5725.2014.04.005
胆汁酸核受体激动剂对瘦素及OB-Rb的影响
Effects of FXR agonist on leptin and OB-Rb
摘要
Abstract
Objective To investigate the effects of GW4064,one FXR agonist,on the leptin and OB-Rb during the differentiation of 3T3-L1 preadipocytes and on the OB-Rb in the HepG2 cells. Methods The mRNA relative expression of leptin , OB-Rb and the protein of leptin on the day of 0 , 2 , 4 , 6 , 8 during the differentiation of 3T3-L1 preadipocytes after interfered with GW4064 were detected by fluorescent real-time PCR and ELISA , respectively. Meanwhile , the mRNA relative expression of OB-Rb of HepG2 cells after treated with GW4064 0 h , 12 h,24 h,48 h were also examined. Results The mRNA relative expression of leptin in the 3T3-L1 preadipocytes and OB-Rb in the HepG2 cells after treated with GW4064 were significantly increased compared with the controlgroup. Also, the protein level of leptin was similar with the mRNA expression. The all differences were statistically significance (P<0.05). However, there was no significant difference on OB-Rb in 3T3-L1 preadipocytes cells (P>0.05). Conclusions GW4064 is able to upregulate the expression of leptin in the 3T3-L1 preadipocytes and OB-Rb in the HepG2 cells. Now the role of leptin in the NAFLD is still unkown, however, the low expression of OB-Rb is related with the leptin resistance in the pathogenesis of NAFLD. So we hypothesize that FXR agonist may treat NAFLD through upreglating the expression of OB-Rb and improving leptin resistance.关键词
胆汁酸核受体/瘦素/OB-Rb/3T3-L1/HepG2Key words
FXR/leptin/OB-Rb/3T3-L1/HepG2引用本文复制引用
辛小敏,张珊珊,钟慕晓,杨公利,彭瑶,朱薇,张亚历..胆汁酸核受体激动剂对瘦素及OB-Rb的影响[J].实用医学杂志,2014,(4):514-517,4.基金项目
广东省科技计划项目 （）
