实用医学杂志Issue(5)：821-823,3.DOI:10.3969/j.issn.1006-5725.2014.05.048
精子染色质结构分析和染色质扩散试验检测精子DNA碎片的比较研究
Comparison of Sperm Chromatin Structure Assay with Sperm Chromatin Dispersion Test for DNA Fragmentation Evaluation in Human Sperm
倪吴花 1金建远 1杨旭 1费前进 1潘承双 1黄学锋1
作者信息
- 1. 325000 温州医科大学附属第一医院生殖医学中心
- 折叠
摘要
Abstract
Objective To assess sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCD) for DNA fragmentation evaluation in human infertility, and the correlation between these two methods. Methods We used SCSA and SCD assays to detect DNA fragmentation in sperm from 134 infertile men. The correlation of SCSA and SCD assays was analyzed. The sperm DNA fragmentation index (DFI) was divided into 3 groups (≤15%DFI, >15~≤30%DFI and>30%DFI), and the difference between SCSA and SCD assays was assessed. Results The SCSA assay was strongly correlated with the SCD assay for sperm DNA fragmentation (r=0.915, P<0.001). There was no significant difference between>15~ ≤30%DFI and>30%DFI groups. However, SCD showed higher levels of DNA fragmentation than that measured by SCSA for≤15%DFI group (13.50 4.82 vs 9.79 2.60, P<0.001). Conclusion There is a strong positive correlation between SCSA and SCD assays in detection of DNA fragmentation. SCD assay showed higher levels of DNA fragmentation than that measured by SCSA for≤15%DFI group.关键词
不育,男(雄)性/精子DNA碎片/精子染色质结构分析/染色体扩散试验Key words
Sperm DNA fragmentation/Sperm Chromatin Structure Assay (SCSA)/Sperm chromatin dispersion test (SCD)引用本文复制引用
倪吴花,金建远,杨旭,费前进,潘承双,黄学锋..精子染色质结构分析和染色质扩散试验检测精子DNA碎片的比较研究[J].实用医学杂志,2014,(5):821-823,3.
