特产研究Issue(4)：10-13,4.
以梅花鹿P21基因为模板比较相对和绝对荧光定量PC R的准确性
Comparative Studies between Relative and Absolute PCR Value Using Sika Deer P21 Gene as an Example
摘要
Abstract
Real time fluorescent quantitative PCR is a highly sensitive and specific nucleic acid analysis technique ,relative and absolute quan-tifications using SYBR Green dye are used widely because of simple operation and low cost .In this study ,we detected P21 gene of sika deer us-ing qPCR andβ-actin gene was used as an internal control .Samples were diluted to different concentrations and the relative and absolute val-ues of P21 andβ-actin genes were determined separately .The results showed that overall absolute quantification was more accurate and more reliable compared to the relative quantification ,as the relative quantification showed a large deviation at × 256 .Both methods showed big devi-ations when the template concentration was very low (lower than 1 000 copes) .Therefore ,we suggest that using absolute quantification whenever possible ,and concentrating samples when the copes target genes are too low .关键词
实时荧光定量PCR/相对定量/绝对定量/P21Key words
real time fluorescent quantitative PCR/relative quantification/absolute quantification/P21分类
生物科学引用本文复制引用
王大涛,郭倩倩,朱宏伟,刘振,王桂武,李春义..以梅花鹿P21基因为模板比较相对和绝对荧光定量PC R的准确性[J].特产研究,2013,(4):10-13,4.基金项目
973前期专项（2011CB111515）；国家自然科学基金（31170950）；吉林省自然科学基金 （）
