生态环境学报Issue(12)：1887-1892,6.
外源和内源呼吸模式下好氧活性污泥的微生物群落演替及生物活性特征
Bacterial community and metabolic characteristics during endogenous and exogenous periods in activated sludge
摘要
Abstract
PCR-DGGE based microbial community analysis combining the determination of the concentration of CODCr, dehydrogenase and total protein was conducted to investigate bacterial community succession and the change of bioactivity in activated sludge fed with or without glucose as carbon resource. Results showed that:in sludge without glucose, bacterial community structure was compariably stable; CODCr concentration was stable at 100 mg·L-1 during the whole incubation of 720 h;dehydrogenase concentration fell to nearly 0 μg·mL-1 from 1 μg·mL-1; amendment with glucose, which raised dehydrogenase concentration to 5μg·mL-1 from 2μg·mL-1, lowered bacterial diversity and caused convergent development on bacterial community structure. Total protein in sludge fed with and without glucose both rose rapidly from 0.05 mg·mL-1 to 0.07 mg·mL-1 at 72 h, then decreased to and kept at 0.01 mg·mL-1 and 0.005 mg·mL-1, respectively. Proteobacteria, following by Bacteroidetes, is the absolutely predominant habitant in sludge under both endogenous and exogenous respiration. Deltaproteobacteria fade away under endogenous respiration over time, while Nitrospirae disappeared under exogenous respiration.关键词
内源呼吸/外源呼吸/好氧活性污泥/微生物群落结构/PCR-DGGEKey words
endogenous respiration/exogenous respiration/activated sludge/bacterial community/PCR-DGGE分类
资源环境引用本文复制引用
罗茜,马宏瑞,朱超,花莉..外源和内源呼吸模式下好氧活性污泥的微生物群落演替及生物活性特征[J].生态环境学报,2013,(12):1887-1892,6.基金项目
国家重点基础研究发展计划（973前期研究专项） （）
