物理化学学报Issue(2)：345-350,6.DOI:10.3866/PKU.WHXB201311282
基于低分子量聚乙烯亚胺和油酸的负电性基因载体
Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid
摘要
Abstract
The development of synthetic vectors based on low-molecular-weight polyethylenimine (PEI) and the construction of negatively charged vectors with high nucleic acid encapsulation are two of the current research trends in gene delivery. In the present study, we developed a stable and negatively charged lipopolyplex based on oleic acid and a low-molecular-weight PEI (2 kDa); it was formed by simultaneously mixing oleic acid micelles and a polyplex produced from PEI (2 kDa) and oligo dsDNA. This lipopolyplex is stable in serum, and because of the low molecular weight of the PEI, the lipopolyplex exhibits very low toxicity. The encapsulation efficiencies of nucleic acids by traditional anionic vectors are low, but very high (more than 80%) by this lipopolyplex. Suitable surface modification with 1,2-distearoryl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethyleneglycol-2000) ammonium salt enabled the lipopolyplex to bind to cells, and a high cellular uptake efficiency (ca 90%) was observed in vitro.关键词
聚乙烯亚胺/油酸/基因传递Key words
Polyethylenimine/Oleic acid/Gene delivery分类
化学化工引用本文复制引用
杨阳,郭霞..基于低分子量聚乙烯亚胺和油酸的负电性基因载体[J].物理化学学报,2014,(2):345-350,6.基金项目
The project was supported by the National Natural Science Foundation of China (21073155,21373179) and NIH grants, USA (CA129835, CA149363, CA151652, CA129421)@@@@国家自然科学基金(21073155,21373179)和美国NIH (CA129835, CA149363, CA151652, CA129421)资助项目 （21073155,21373179）
