西安交通大学学报（医学版）Issue(3)：375-379,5.DOI:10.7652/jdyxb201403020
Cystatin M抑制胃癌SGC-7901细胞的增殖和迁移
Cystatin M inhibits the proliferation and migration of gastric cancer SGC-7901 cell lines
摘要
Abstract
Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector,and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells.Methods There were three groups in the experiment:pcDNA3.1(+)-CST6 group,pcDNA3.1 (+)group and non-transfected group.RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells.The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay,respectively.Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein.The proliferation and migration of pcDNA3 .1 (+)-CST6 group cells were reduced compared with those of cells in the other two groups (P<0.05).Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.关键词
CST6 基因/Cystatin M/胃癌/SGC-7901 细胞/增殖/迁移Key words
CST6 gene/cystatin M/gastric cancer/SGC-7901 cell/proliferation/migration分类
医药卫生引用本文复制引用
赵亚敏,何炜,王峰,赵国强,叶延伟,樊青霞..Cystatin M抑制胃癌SGC-7901细胞的增殖和迁移[J].西安交通大学学报（医学版）,2014,(3):375-379,5.基金项目
河南省国际科技合作计划项目(No.12430510009) （No.12430510009）
国家自然科学基金资助项目(No.81201955) Supported by the Health Department Foundation of Henan Province (No.12430510009)and the National Natural Science Foundation of China(No.81201955) （No.81201955）
