西安交通大学学报（医学版）Issue(3)：385-389,5.DOI:10.7652/jdyxb201403022
种植窗期子宫内膜自然杀伤细胞对内膜血管生成的影响及子宫动脉血流与体外受精胚胎移植结局的相关性
Effect of uterine natural killer cells in the window endometrium of implantation on angiogenesis and relationship between uterine artery pulsation index and IVF treatment outcome
摘要
Abstract
Objective To investigate the effect of uterine natural killer (uNK)cells in the endometrium of implantation window stage on endometrial angiogenesis and the relationship between uterine artery pulsation index and in vitro fertilization (IVF)treatment outcome.Methods We recruited 30 patients who all had received IVF and embryo transfer (IVF-ET)at the age of less than 3 5 years.Uterine artery pulsation indexes were assessed by vaginal color Doppler, and the endometrium in the window of implantation was collected. Expression and localization of CD5 6+,uNK cells marker;F8 factor,vascular endothelial cell marker;alpha smooth muscle actin (αSMA ), vascular smooth muscle cell marker;and heavy chain smooth myosin (SMM ) were detected by immunohistochemical SP method.The patients were divided into pregnancy group and repeated implantation failure group based on assisted pregnancy outcome.Results ① The positive expression rates of uNK cells and F8 factor were positively related (r=0 .5 8 1 ,P<0 .0 1 ).② The positive expression rate of uNK cells was positively related to that ofαSMA and SMM (r=0 .5 7 2 ,r=0 .5 6 9 ,P<0 .0 1 ).③ Uterine artery pulsation index was 0 .8 5 ± 1 .5 1 in repeated implantation failure group and 0.93±0.24 in pregnancy group,without any significant difference between the two groups (t=-0.98,P>0.05).Conclusion ① uNK cells in the window of implantation are positively correlated with microvascular density and vascular maturity, indicating that they play an important role in angiogenesis of the endometrium.② The effect of uterine artery dynamic index on embryo transfer outcome does not differ significantly between pregnancy group and repeated implantation failure group.Further study is needed.关键词
uNK细胞/种植窗期/F8 因子/a-平滑肌肌动蛋白(aSMA)/重链肌球蛋白(SMM)/体外受精Key words
uterine natural killer (uNK)cell/window of implantation/F8 factor/alpha smooth muscle actin (aSMA)/heavy chain smooth myosin (SMM)/in vitro fertilization分类
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赵静,罗莉,王卉芳,闫春芳,李东红,王蕴颖,张平,王丽萍,蔡凤梅..种植窗期子宫内膜自然杀伤细胞对内膜血管生成的影响及子宫动脉血流与体外受精胚胎移植结局的相关性[J].西安交通大学学报（医学版）,2014,(3):385-389,5.基金项目
陕西省科技攻关项目(No.2010k15-06-05) Supported by the Key Science and Technology Program of Shaanxi Province (No.2010k15-06-05) （No.2010k15-06-05）
