西安交通大学学报（医学版）Issue(3)：411-414,4.DOI:10.7652/jdyxb201403027
赖氨大黄酸对快速老化小鼠SAMP 10肾组织TNF-α、IL-6、NF-κB表达的影响
Effects of rhein lysinate on the expressions of TNF-α,IL-6 and NF-κB in the kidney tissue of SAMP 1 0 mice
摘要
Abstract
Objective To investigate the effects of rhein lysinate (RHL)on the expressions of TNF-α,IL-6 and NF-κB in the kidney tissue of senescence accelerated mouse prone 10 (SAMP 10)mice.Methods We selected 1 8 male mice (SAMP 1 0 )aged 7 months for the study and randomly divided them into blank control group and groups of different concentrations of RHL;six senescence accelerated mouse resistance 1 (SAMR 1 )served as the young control group.After 6 weeks’ treatment,HE staining was used to detect the pathological changes of the kidney.The expressions of TNF-α,IL-6 and NF-κB at the protein level were detected by immunohistochemistry and Western blotting.Results RHL treatment did not affect the body weight of SAMP 10 mice (P>0.05 ). Compared with SAMR 1 mice, contracted and destroyed renal glomeruli and infiltration of mononuclear macrophages were observed in control SAMP10 mice.However,this pathological process was blocked by RHL (25 mg/kg and 50 mg/kg ) treatments. In addition, the overexpressions of TNF-α, IL-6 and NF-κB and the phosphorylation of NF-κB in the kidney tissue of SAMP 10 mice could be inhibited by RHL treatments (P<0.05). Conclusion RHL inhibits the inflammatory reaction of the kidney tissue,which may be one of the mechanisms by which RHL exerts its kidney-protecting and anti-aging effects.关键词
赖氨大黄酸(RHL)/SAMP 10 快速老化小鼠/肾小球肾炎/肿瘤坏死因子-α/白细胞介素6(IL-6)/核因子κB (NF-κB)/抗衰老Key words
rhein lysinate/senescence accelerated mouse prone 1 0 (SAMP 1 0 )/glomerulonephritis/tumor necrosis factor-alpha (TNF-α)/IL-6/NF-κB/anti-aging分类
医药卫生引用本文复制引用
甄永占,胡刚,赵毓芳,李冉,章广玲,朱丽华,林雅军..赖氨大黄酸对快速老化小鼠SAMP 10肾组织TNF-α、IL-6、NF-κB表达的影响[J].西安交通大学学报（医学版）,2014,(3):411-414,4.基金项目
国家自然科学基金资助项目(No.81001439) Supported by the National Natural Science Foundation of China (No.81001439) （No.81001439）
