陕西科技大学学报（自然科学版）Issue(1)：128-132,147,6.
纤维堆囊菌产埃博霉素B的发酵条件优化
Optimization of fermentation conditions for epothilone B produced by sorangium cellulosum
摘要
Abstract
Epothilone B is a novel anti-cancer macrolide compound produced by sorangium cel-lulosum and show s the excellent activity against tumor both in antitumor spectra and in effect .This paper focused on the optimization of fermentation conditions of epothilone B pro-duced by sorangium cellulosum SoF5-76 .One-variable-at-a-time design and orthogonal exper-iments were employed to obtain the optimum fermentation conditons .The results were as follows :initial pH of fermentation medium 7 .4 ,medium volume 50/250 mL ,shaker revolu-tion 200 r/min ,seed age 60 h ,inoculation size 10% culture temperature 30 ℃ ,fermentation period 6 d .Under the optimized conditions ,the epothilone B production reached at 36 .57 mg/L and was 22 .1% higher than preliminary fermentation conditions .关键词
纤维堆囊菌/埃博霉素B/发酵条件优化/正交试验Key words
sorangium cellulosum/epothilone B/optimization of fermentation conditions/or-thogonal experiment分类
药学引用本文复制引用
龚国利,王娜,刘丽丽,游银伟..纤维堆囊菌产埃博霉素B的发酵条件优化[J].陕西科技大学学报（自然科学版）,2014,(1):128-132,147,6.基金项目
国家自然科学基金项目（20906058）；陕西科技大学青年学术骨干培养计划项目 （）
