中国组织工程研究Issue(40)：7084-7089,6.DOI:10.3969/j.issn.2095-4344.2013.40.010
异丙肾上腺素影响下颌下腺干/祖细胞增殖的数量还是能力？
Isoproterenol influence on stem/progenitor cells of submandibular glands:Proliferative number or capability?
摘要
Abstract
BACKGROUND:Injection of isoproterenol is known to induce proliferation and hypertrophy of acinar cells in rodent salivary glands. However, the clonal proliferation ability of stem/progenitor cells of salivary glands by isoproterenol remains unclear. <br> OBJECTIVE:To study the proliferation and activation ability of stem/progenitor cells of submandibular gland with colony assay by intraperitoneal injection of isoproterenol. <br> METHODS:Sprague-Dawley rats were randomly divided into two groups, isoproterenol and control groups, respectively intraperitonal y injected with isoproterenol and normal saline for 5 consecutive days. The gland tissues were harvested, and the stem/progenitor cells of submandibular gland were obtained by enzyme digestion in vitro. The number of clonal colonies of each group was analyzed. The larger colony cells were col ected for immunohistochemistry staining with CD90.1, laminin andα6β1. <br> RESULTS AND CONCLUSION:The number of middle and low proliferative potential colony-forming cells was less but high proliferative potential colony forming cells were significantly more in isoproterenol group compared with control group (P<0.05). However, there was no significant difference in the total number of the colonies between two groups (P>0.05). The high proliferative potential colony forming cells were positive for CD90.1, laminin andα6β1. Results showed that isoproterenol treatment model cannot increase the cellnumber, but enhance the proliferation ability of stem/progenitor cells from the submandibular gland.关键词
干细胞/干细胞培养与分化/异丙肾上腺素/下颌下腺/增殖/肥大/干/祖细胞/集落分析/省级基金/干细胞图片文章分类
医药卫生引用本文复制引用
唐岳鹏,黄桂林,姚礼,张霓霓,易杰..异丙肾上腺素影响下颌下腺干/祖细胞增殖的数量还是能力？[J].中国组织工程研究,2013,(40):7084-7089,6.基金项目
贵州省科技厅科技攻关项目基金(SY[2011]3016)；贵州省科学技术基金(黔科合J字LKZ[2011]23)@@@@the Science and Technology Development Program of Guizhou Science and Technology Department, No. SY[2011]3016 （SY[2011]3016）
the Science and Technology Foundation of Guizhou Province, No. LKZ[2011]23 （）
