中国组织工程研究Issue(41)：7248-7254,7.DOI:10.3969/j.issn.2095-4344.2013.41.010
急性脊髓损伤后差异蛋白的表达
Differential protein expression after acute spinal cord injury
摘要
Abstract
BACKGROUND:In mass spectrometry analysis, the same protein in different samples labeled with isobaric tags for relative and absolute quantitation presents the same mass-to-charge ratio, while in the tandem mass spectrometry analysis, the ion signal shows different mass-to-charge ratio (114-121). Thus the quantitative information of the related proteins can be obtained. OBJECTIVE:To establish the protein spectrum of spinal cord tissue differences proteins after acute spinal cord injury, and to explore the spinal differential protein expression on the molecular level using isobaric tags for relative and absolute quantitation combined with LC-MS/MS mass spectrometry technique. METHODS:Eight Sprague Dawley rats were selected to establish the acute spinal cord injury models using Al en’s method. The rats were randomly divided into 0 hour spinal cord injury group and 8 hours spinal cord injury group, four rats in each group. The spinal cord tissues were col ected after injury, and the spinal cord tissue differences proteins were analyzed with isobaric tags for relative and absolute quantitation technique after acute spinal cord injury. RESULTS AND CONCLUSION:Total y 220 differential y expressed proteins were identified in this research, the number of up-regulation proteins was 116 and the number of down-regulation proteins was 104. There were 12 differential proteins related to neural regeneration, and among the 12 proteins, there were seven up-regulation proteins and five down-regulation proteins. The various identified differential proteins and significantly expressed nerve growth factors in this experiment can be used as the biomarkers of acute spinal cord injury or used as the strong evidence for the clinical management and monitoring of the injury process and target therapy of acute spinal cord injury, as wel as the effect evaluation.关键词
组织构建/组织构建细胞学实验/急性脊髓损伤/神经再生/蛋白质组学/同位素标记相对和绝对定量/质谱分析/神经生长因子/国家自然科学基金Key words
spinal cord injuries/nerve regeneration/proteomics/mass spectrometry分类
医药卫生引用本文复制引用
徐磊磊,周义军,宋兴华,丁俐文,陈江涛,王翀,甘雨灵,朱晓萌..急性脊髓损伤后差异蛋白的表达[J].中国组织工程研究,2013,(41):7248-7254,7.基金项目
国家自然科学基金(81160218/H0605)“不同时间点鞘内注射甲基强的松龙治疗大鼠急性脊髓损伤的蛋白组学实验研究” （81160218/H0605）
