中国组织工程研究Issue(43)：7587-7593,7.DOI:10.3969/j.issn.2095-4344.2013.43.014
椎间盘突出患者椎间盘Fas基因的表达
Fas gene expression of intervertebral disc in the patients with intervertebral disc herniation
摘要
Abstract
BACKGROUND:The clinical research have found that the interbervebral disc herniation often occurs in several members or even al the members of a family, and the location, reason and symptom are basical y the same, indicating that genes play an important role in this kind of disease. OBJECTIVE:To analyze the apoptosis Fas gene expression characteristics of lumbar disc in the familial patients with intervertebral disc herniation. METHODS:Semi-quantitative reverse transcription-PCR was used to test Fas gene expression of vertebral pulp and cartilage endplate in the intervertebral disc among 15 familial patients, 21 ordinary patients and five fresh cadavers. RESULTS AND CONCLUSION:Fas gene expression level of endplate of familial and ordinary patients with intervertebral disc herniation was higher than that of fresh cadavers, and there was no significant difference (P<0.05);there was no significant difference in Fas gene expression in endplates between familial patients and ordinary patients with intervertebral disc herniation (P>0.05). Compared with the vertebral pulps of ordinary patients with intervertebral disc herniation and fresh cadavers, there was no significant difference in the Fas expression of vertebral pulps of familial patients with intervertebral disc herniation (P>0.05). The increasing Fas gene expression may be secondary in the endplates of familial patients with intervertebral disc herniation, which can prevent intervertebral disc degeneration through preventing the endplate degeneration.关键词
骨关节植入物/脊柱损伤基础实验/腰椎/椎间盘/椎间盘突出/Fas/基因/表达/终板/髓核/省级基金Key words
lumbar vertebrae/intervertebral disk/imtervertebral disk displacement/antigens, CD95/gene expression/reverse transcriptase polymerase chain reaction分类
医药卫生引用本文复制引用
吕浩然,杨进顺,黄彦,赵玉,冯善伟..椎间盘突出患者椎间盘Fas基因的表达[J].中国组织工程研究,2013,(43):7587-7593,7.基金项目
广东省人口计生委科研基金项目(20110325)，腰椎间盘退变Fas基因突变的筛查及其相关研究；广东省医学科研基金项目(A2011264)，三维导航下脊椎微创技术及相关工具的研制；广东省科技厅基金项目(2011B031800251)，实时术中三维导航在骨科颈椎内固定微创技术中的应用研究。@@@@Scientific Research Fund Project of Guangdong Provincial Population and Family Planning Commission, No.20110325 （20110325）
Guangdong Medical Research Foundation, No. A2011264 （）
Project of Guangdong Provincial Science and Technology Department, No.2011B031800251 （）
