中国组织工程研究Issue(45)：7855-7860,6.DOI:10.3969/j.issn.2095-4344.2013.45.006
CM-DIL和DAPI标记的骨髓间充质干细胞
Comparison of CM-DIL and DAPI labeled bone marrow-derived mesenchymal stem cells
摘要
Abstract
BACKGROUND:cellmarker technology has been widely applied in many studies concerning celltransplantation. Chlormethylbenzamido-1,1-dioctadecyl-3,3,3’3’-tetramethylin-docarbocyamine (CM-DIL) and 4’,6-diamidino-2-phenylindole (DAPI) are commonly used for labeling cells. To our knowledge, there are few reports on comparing the two fluorescent dyes. <br> OBJECTIVE:To compare the effects of CM-DIL and DAPI on labeling bone marrow mesenchymal stem cells in vitro and in vivo. <br> METHODS:Isolation and expansion of bone marrow-derived mesenchymal stem cells were performed according to attachment culture. The cells were labeled by CM-DIL and DAPI, respectively. cellviability was assessed via trypan blue exclusion assay. Growth curves of bone marrow-derived mesenchymal stem cells were depicted using MTS assay. The reduction of fluorescent intensity was observed under an inverted fluorescent contrast phase microscope from passage 1 to passage 3 after labeling. Myocardial infarction was induced by left anterior artery ligation in Sprague-Dawley rats. One week later, bone marrow-derived mesenchymal stem cells labeled by CM-DIL or DAPI were injected randomly into the border area of infarct myocardium. After 3 days, transplanted celldistribution was examined under the fluorescent microscope through paraffin sections and frozen sections respectively. <br> RESULTS AND CONCLUSION:In vitro, bone marrow-derived mesenchymal stem cells labeled by both CM-DIL and DAPI showed decreased cellproliferation during the early period;the percentage of fluorescent-positive cells was approximately 100%in the two groups;however, the fluorescent intensity was significantly reduced from passage 1 to passage 3 in bone marrow-derived mesenchymal stem cells labeled by DAPI. In vivo, the transplanted cells were detected in a concentrated way both on the paraffin sections and frozen ones;the background color of frozen sections was lower in the CM-DIL group than in the DAPI group;false positive results of fluorescent expression could be eliminated in the CM-DIL group by using fluorescent mounting medium with the fluorescence of DNA staining. These data indicates that CM-DIL is more appropriate to in vivo tracing cells than DAPI.关键词
干细胞/骨髓干细胞/CM-DIL/DAPI/荧光染料/标记/骨髓间充质干细胞/细胞活力/细胞增殖/心肌梗死/荧光表达/干细胞图片文章分类
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商青青,李凯,周建业,胡盛寿..CM-DIL和DAPI标记的骨髓间充质干细胞[J].中国组织工程研究,2013,(45):7855-7860,6.
