中国组织工程研究Issue(45)：7885-7890,6.DOI:10.3969/j.issn.2095-4344.2013.45.010
碱性成纤维细胞生长因子基因转染优化脐带间充质干细胞的培养
Effects of basic fibroblast growth factor gene transfection on the proliferation of umbilical cord mesenchymal stem cells
郑颖娟 1田建昌2
作者信息
- 1. 解放军第三七一中心医院肿瘤血液科，河南省新乡市 453000
- 2. 解放军第三七一中心医院心胸外科，河南省新乡市 453000
- 折叠
摘要
Abstract
BACKGROUND:At present, exogenous basic fibroblast growth factor gene can be transfected into umbilical cord mesenchymal stem cells via a recombinant adeno-associated virus vector and exhibit sustained expression in transfected cells. This method can regulate cellproliferation and directed differentiation to obtain efficient long-lasting therapeutic effects. <br> OBJECTIVE:To investigate the effects of basic fibroblast growth factor gene transfection via a recombinant adeno-associated virus vector on the proliferation and cellcycle of human umbilical cord mesenchymal stem cells cultured in vitro. <br> METHODS:Human umbilical cord mesenchymal stem cells were cultured by the suspension culture in vitro, and were transfected by recombinant adeno-associated virus-mediated basic fibroblast growth factor gene. Cultured cells were divided into three groups:control group, basic fibroblast growth factor group, and recombinant adeno-associated virus group. Reverse transcription-PCR and western blot were used to assess the knockdown efficiency. cellular proliferation was determined by cellgrowth curve and cellCounting Kit-8 assay. The cellcycle was analyzed by flow cytometry. <br> RESULTS AND CONCLUSION:Compared with the other two groups, the expression of basic fibroblast growth factor mRNA and protein increased significantly, the cellgrowth speed was also significantly increased, the cellcycle of G0/G1 phase was decreased and cellnumber in S phase was increased in the basic fibroblast growth factor group after transfection. These findings suggest that the recombinant adeno-associated virus-mediated basic fibroblast growth factor gene can promote the proliferation of umbilical cord mesenchymal stem cells proliferation cultured in vitro, and also can optimize the cellculture.关键词
干细胞/脐带脐血干细胞/脐带/间充质干细胞/碱性成纤维细胞生长因子/基因转染/腺病毒/干细胞图片文章分类
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郑颖娟,田建昌..碱性成纤维细胞生长因子基因转染优化脐带间充质干细胞的培养[J].中国组织工程研究,2013,(45):7885-7890,6.
