中国组织工程研究Issue(46)：7988-7993,6.DOI:10.3969/j.issn.2095-4344.2013.46.002
白细胞介素1受体相关激酶4基因沉默抑制人成骨样细胞相关基因的表达
Inhibitory effect of interleukin-1 receptor-associated kinase-4 silencing on mitogen-activated protein kinases expression in human osteoblast-like cells
摘要
Abstract
BACKGROUND:The molecular mechanism of periprosthesis osteolysis is not yet completely clear. Periprosthetic osteolysis and absorption is the pathological and physiological process typical of artificial joint loosening. Interleukin-1 can affect bone resorption process through a mitogen-activated protein kinases (MAPK) signaling pathway. <br> OBJECTIVE:To explore the effects of siRNA-induced interleukin-1 receptor-associated kinase-4 gene (IRAK-4) silence on MAPK expression in MG63 cells, which may provide experimental basis for treatment and prevention of periprosthesis osteolysis. <br> METHODS:The siRNA sequences of the target gene, IRAK-4, were constructed and transferred into MG63 cells using Lipofectamine 2000. There were three groups:blank group=MG63 cells, control group=MG63 cells transfected with scrambled IRAK-4siRNA, and silence group=MG63 cells transfected with specific IRAK-4 siRNA. The protein level of extracellular regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases (p38MAPK) were detected by western blot assay. <br> RESULTS AND CONCLUSION:The expression of IRAK-4 mRNA and protein in the silence group was significantly decreased compared with the control group. Compared with the blank and control groups, 48 hours after the transfection, IRAK-4 gene silencing in MG63 cells decreased protein expression of p-JNK1/2P46, p-ERK1/2 and p-p38MAPK (P<0.05). IRAK-4 silencing inhibited ERK, JNK and p38MAPK expression in osteoblast-like cells.关键词
组织构建/骨组织构建/假体周围骨溶解/无菌性松动/人成骨样细胞/白细胞介素1受体相关激酶4/RNA干扰/基因沉默/基因转染/促分裂素原活化蛋白激酶/国家自然科学基金分类
医药卫生引用本文复制引用
杨子波,黄保丁,向珊珊,邬培慧,张志奇,廖威明..白细胞介素1受体相关激酶4基因沉默抑制人成骨样细胞相关基因的表达[J].中国组织工程研究,2013,(46):7988-7993,6.基金项目
国家自然科学基金项目(81171709)@@@@the National Natural Science Foundation of China, No.81171709 （81171709）
