中国组织工程研究Issue(46)：8018-8023,6.DOI:10.3969/j.issn.2095-4344.2013.46.007
高压氧预处理损伤脊髓Bcl-2/Bax的表达
Effect of hyperbaric oxygen preconditioning on expression of Bcl-2 and Bax after spinal cord injury
张玉强 1李游 2曹阳 1董明岩 1吕刚1
作者信息
- 1. 辽宁医学院附属第一医院骨科，辽宁省锦州市 121000
- 2. 辽宁医学院附属第一医院眼科，辽宁省锦州市 121000
- 折叠
摘要
Abstract
BACKGROUND:Studies have shown that hyperbaric oxygen preconditioning can reduce neuronal apoptosis <br> and improve neurologic dysfunction after spinal cord injury. However, the mechanism of inhibiting apoptosis is stil unclear. <br> OBJECTIVE:To study the effect and significance of hyperbaric oxygen preconditioning on expression of Bcl-2 and Bax protein after spinal cord injury in rats. <br> METHODS:Sixty-six male adult Sprague-Dawley rats were divided into sham operation group, model group, treatment group at random. Animals in the sham group were six, and animals in the model and treatment groups were 30, respectively. Animals in the treatment group received hyperbaric oxygen preconditioning at 2.5 MPa (100%O 2 ), once for 1 hour and total y five times within 10 days before establishment of spinal cord injury models. In the sham operation group, only laminectomy was done. In the model group, a spinal cord injury model was prepared using Al en’s method. RESULTS AND CONCLUSION:The results of immunochemistry indicated that there were few Bcl-2 and <br> Bax-positive cells in the sham operation group;a number of Bcl-2 and Bax-positive cells were visible in the model and treatment groups. Bcl-2 and Bax expressions reached the peak at day 7 and 3, respectively, after injury. After treatment with hyperbaric oxygen preconditioning, Bcl-2 positive cells decreased significantly, while Bax-positive cells decreased significantly (P<0.05). It indicates that the hyperbaric oxygen preconditioning can increase Bcl-2 expression but decrease Bax expression.关键词
组织构建/组织构建与生物活性因子/高压氧预处理/高压氧/脊髓损伤/Bcl-2/Bax/免疫组化/脊髓分类
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张玉强,李游,曹阳,董明岩,吕刚..高压氧预处理损伤脊髓Bcl-2/Bax的表达[J].中国组织工程研究,2013,(46):8018-8023,6.
