中国组织工程研究Issue(46)：8049-8055,7.DOI:10.3969/j.issn.2095-4344.2013.46.012
核磁共振氢谱代谢组学方法确定X射线对小鼠辐射的损伤
Radiation metabolomics of minimally invasive urine biomarkers for X-ray radiation exposure in mice
摘要
Abstract
BACKGROUND:Single hematology analysis can only reflect the body injury at a certain time point after radiation damage, but cannot reflect the longer-term cumulative status after radiation damage. OBJECTIVE:To identify the biomarkers in blood and urine in mice after radiation damage with metabolomics method based on nuclear magnetic resonance hydrogen spectrum. METHODS:Forty-eight mice were randomly divided into four groups and received 0 (sham radiation), 3, 9 and 27 Gy radiation. The blood samples were col ected at 24 hours and 5 days after radiation. Another 36 mice were col ected and divided into three groups and received 0 (sham radiation), 9 and 27 Gy radiation, then the urine samples were col ected at 2 days before radiation and 5 days after radiation for 24 hours. The blood and urine samples were analyzed with proton nuclear magnetic resonance spectroscopy. <br> RESULTS AND CONCLUSION:The content of aspartic aminotransferase and alanine aminotransferase in blood plasma maintained a stable level after 3-or 9-Gy X-ray radiation, but the level of alkaline phosphatase in blood plasma was increased significantly after 9-Gy radiation, which indicating that low-dose head radiation may cause increased radiation damage and repair. The level of total superoxide dismutase in blood plasma was significantly decreased at 5 days after radiation which indicating that head radiation in mice could cause systemic oxidative stress. Meanwhile, the N-hexamolglycine and-thymidine level in the urine samples was significantly increased after different doses X-ray radiation, which can be used as the radiation damage markers in urine samples after head radiation. The 3-hydroxy-2-methylbenzoic acid 3-O-sulfate level in urine samples was increased by 2.5 times after received 9-Gy radiation, which can be used as the specific markers of middle-and low-dose radiation damage;the level of taurine in the urine samples was increased by 20%after 27-Gy radiation, which can be used as the specific markers of high-dose radiation damage.关键词
组织构建/组织构建细胞学实验/代谢组学/X射线/核磁共振氢谱/辐射损伤/碱性磷酸酶/超氧化物歧化酶/生物标志物/国家自然科学基金分类
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王敏,潘晓婧,刘斌,张红..核磁共振氢谱代谢组学方法确定X射线对小鼠辐射的损伤[J].中国组织工程研究,2013,(46):8049-8055,7.基金项目
国家重点基础研究发展计划资助(2010CB834202)；国家自然科学基金重点项目(10835011)；国家自然科学基金面上项目(30770639)；兰州大学中央高校基本科研费2012年面上项目(lzujbky-2012-154)；兰州大学口腔医学院扶持基金项目(120653)@@@@Major State Basic Research Development Program of China, No.2010CB834202 （2010CB834202）
Key Program of （）
