中国组织工程研究Issue(46)：8062-8068,7.DOI:10.3969/j.issn.2095-4344.2013.46.014
小干扰RNA对人类风湿关节炎患者滑膜细胞基因表达的影响
Effect of small interfering RNA on gene expression of synovial cells in patients with rheumatoid arthritis
侯春凤 1孙闵 2李树杰 1赵建宏 1王吉波3
作者信息
- 1. 济宁市第一人民医院风湿免疫科，山东省济宁市 272002
- 2. 济宁医学院临床学院，山东省济宁市 272013
- 3. 青岛大学附属医院风湿免疫科，山东省青岛市 266071
- 折叠
摘要
Abstract
BACKGROUND:The etiological factor for rheumatoid arthritis remains unclear, but the effects of nuclear factor-κB on the onset of rheumatoid arthritis have been gradual y paid great attention by rheumatologists. <br> OBJECTIVE:By using the RNA interference (RNAi) technique to block the signal pathway of nuclear factor-κB <br> mRNA of human rheumatoid arthritis synovial cells, this study explored its application prospect in the treatment of rheumatoid arthritis. <br> METHODS:The synovial cells were isolated, digested, and cultured for further use. In accordance with the <br> design principle of smal interfering RNA (siRNA), target sequences of siRNA of nuclear factor-κB were identified, and siRNA expression vector of nuclear factor-κB was synthesized and constructed. The four pGenesil-1/nuclear factor-κB siRNA expression vectors were transfected into the first passage of synovial cells that wel grew. Blank and negative control groups were set. cells at 12, 24, 48, 72 hours, 5 and 7 days after transfection were col ected, and RNA was extracted. Intracellular nuclear factor-κB mRNA expression levels were measured, and siRNA plasmid vector that could effectively inhibit nuclear factor-κB mRNA expression was screened out. <br> RESULTS AND CONCLUSION:Nuclear factor-κB highly expressed in synovial cells after human rheumatoid arthritis. 3#pGenesil-1/nuclear factor-κB apparently suppressed nuclear factor-κB mRNA expression in synovial cells with human rheumatoid arthritis. RNAi technique blocked nuclear factor-κB mRNA expression. Therefore, the block of nuclear factor-κB signal pathway might be a good target for rheumatoid arthritis gene therapy.关键词
组织构建/组织构建细胞学实验/关节炎/类风湿/核转录因子κB/pGenesil-1表达载体/RNA干扰/实时荧光定量聚合酶链反应分类
医药卫生引用本文复制引用
侯春凤,孙闵,李树杰,赵建宏,王吉波..小干扰RNA对人类风湿关节炎患者滑膜细胞基因表达的影响[J].中国组织工程研究,2013,(46):8062-8068,7.
