芒果苷对缺氧损伤骨髓间充质干细胞凋亡的保护
Mangiferin protects bone marrow-derived mesenchymal stem cells against hypoxia-induced apoptosis
摘要
Abstract
BACKGROUND:Hypoxic death limits application of cells in transplantation and tissue regeneration. <br> OBJECTIVE:To investigate the protective effects of mangiferin on bone marrow-derived mesenchymal stem cells against hypoxia injury-induced apoptosis resulted from cobalt chloride. <br> METHODS:Rat bone marrow-derived mesenchymal stem cells were in vitro cultured and hypoxia cellmodel was established by cobalt chloride. Model cells were treated with mangiferin. Protective effects of mangiferin were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;cellapoptosis and mitochondrial membrane potential were detected using flow cytometry. <br> RESULTS AND CONCLUSION:Cobalt chloride significantly inhibited growth of bone marrow-derived mesenchymal stem cells in a dose-dependent manner. The apoptosis rate of cells was (42.49±3.96)%after treated with 200μmol/L cobalt chloride for 12 hours, (46.37±4.49)%after treated for 24 hours. With increasing concentration of mangiferin, apoptosis of bone marrow-derived mesenchymal stem cells in hypoxic model was gradual y reduced (P<0.01), indicating that mangiferin has a protective effect in a concentration-dependent manner on rat bone marrow-derived mesenchymal stem cells in hypoxic injury. Cobalt chloride can induce hypoxic model successful y in bone marrow-derived mesenchymal stem cells. There are some advantages of accurate dose control, no special equipment requirements, and easy operation. Mangiferin can effectively inhibit bone marrow-derived mesenchymal stem cells apoptosis under hypoxic injury.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/缺氧/凋亡/芒果苷/氯化钴/省级基金/干细胞图片文章分类
医药卫生引用本文复制引用
李晓峰,罗世兴,赵劲民,程建文,谭桢..芒果苷对缺氧损伤骨髓间充质干细胞凋亡的保护[J].中国组织工程研究,2013,(49):8481-8487,7.基金项目
广西卫生厅中医药科技专项(GZKZ 10-011)；广西研究生教育创新项目(2010105981002D26)；高等学校博士学科点专项科研基金资助项目(20104503110002)；广西科技厅人口健康与食品安全关键技术研究开发项目(桂科攻1140003A-31)；广西卫生厅自筹经费科研课题(Z2012059)；广西教育厅立项项目(桂教201010LX050)@@@@the Science and Technology Program for Traditional Chinese Medicine, Department of Health of Guangxi Zhuang Autonomous Region, No. GZKZ 10-011 （GZKZ 10-011）
Post-graduate Education Innovation Program of Guangxi Zhuang Autonomous Region, No.2010105981002D26 （）
the Scientific Research Foundation for Doctors of High Institutes, No.20104503110002 （）
the Development Program for the Key Technology of Population Health and Food Safety, Department of Science and Technology, Guangxi Zhuang Autonomous Region, No.1140003A-31 （）
the Scientific Research Program of Self-provided Funds of Department of Science and Technology, Guangxi Zhuang Autonomous Region, No. Z2012059 （）
the Program from the Department of Education of Guangxi Zhuang Autonomous Region, No.201010LX050 （）
