中国组织工程研究Issue(49)：8608-8614,7.DOI:10.3969/j.issn.2095-4344.2013.49.023
诱导多能干细胞与细胞重编程技术的研究与未来
Induced pluripotent stem cells and cell reprogramming technologies
摘要
Abstract
BACKGROUND:At present, the development of reprogramming technology provides a wide prospect for stem cellresearch. Through the ectopic co-expression of reprogramming factors, the somatic cells can be reprogrammed to a pluripotent state, termed as induced pluripotent stem cells, which can avoid the ethical controversy faced in the research and application of embryonic stem cells. Also, we can generate patient-specific and disease-specific induced pluripotent stem cells, which significantly decrease immuno-rejection. However, reprogramming technology faces some chal enges, such as low efficiency and safety. <br> OBJECTIVE:Based on the characteristics of induced pluripotent stem cells and the principles of reprogramming, to detail the progress in reprogramming technology from five aspects, including cellresources, carriers, transcription factors, microRNA and signal transduction pathway. <br> METHODS:A computer-based online retrieval was performed to search papers published form January 1990 to April 2013 in VIP periodical ful-text database, Wanfang periodical ful-text database, CNKI periodical ful-text database, PubMed database and Springer database with key words of“reprogramming, induced pluripotent stem cell, signal transduction pathway, epigenetics, microRNA, transcription factor, vector, somatic cell, smal molecule compound, safety”both in Chinese and English. After excluding objective-independent papers, 67 papers were included for further analysis. <br> RESULTS AND CONCLUSION:By exploring different cellresources, different carriers, various combination of transcription factors, microRNAs or inhibition of the signal transduction pathways, the reprogramming efficiency and safety have been improved greatly. However, currently, induced pluripotent stem cells stil could not meet the requirement of clinical application. To achieve the clinical application of induced pluripotent stem cells, it is urgent to explore the mechanism of reprogramming, and to optimize the programming strategy.关键词
干细胞/干细胞综述/重编程/诱导多能干细胞/信号转导通路/表观遗传/microRNA/转录因子/载体/体细胞/小分子化合物/安全性/973项目分类
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赵星,黄元华,马燕琳..诱导多能干细胞与细胞重编程技术的研究与未来[J].中国组织工程研究,2013,(49):8608-8614,7.基金项目
国家科技部973项目(2012CB966502)；海南省科技厅国际合作重点项目(GJXM20100004，GJXM201106)；人力资源和社会保障部2011年度留学人员科技活动择优资助项目(201150804)；海南省自然科学基金项目(812185)；海口市重点科技计划项目(2012078)@@@@the National Basic Research Program of China (973 Program), No.2012CB966502 （2012CB966502）
Key International Cooperation Program of Science and Technology Department of Hainan Province, No. GJXM20100004, GJXM201106 （）
Merit-funded Scientific Research Projects for the Overseas Students in 2011, Ministry of Human Resources and Social Security of China, No.201150804 （）
Natural Science Foundation of Hainan Province, No.812185 （）
Key Science and Technology Plan Program of Haikou City, No.2012078 （）
