中国组织工程研究Issue(1)：100-105,6.DOI:10.3969/j.issn.2095-4344.2014.01.017
软骨细胞上清液诱导滑膜间充质干细胞微团培养成软骨
Chondrocyte supernatant induces chondrogenesis and pellet cultivation of rat synovial mesenchymal stem cells
摘要
Abstract
BACKGROUND:Synovial mesenchymal stem cells have the ability of multilineage differentiation in vitro, which are expected to be seed cells for the treatment of cartilage defects in cartilage tissue engineering. Appropriate growth factors are critical for the chondrocyte differentiation of synovial mesenchymal stem cells. <br> OBJECTIVE:To study the role of secreted factors by chondrocytes to induce chondrogenesis of synovial mesenchymal stem cells. <br> METHODS:The synovial mesenchymal stem cells and chondrocytes were harvested from rat knee joints and cultured through the digestion method. The supernatant was col ected from chondrocytes, and centrifuged, filtered and cryopreserved. The third passage synovial mesenchymal stem cells centrifuged as pel ets were cultured in the chondrocyte supernatant for 21 days. And the cells morphology was examined and the type II col agen and aggrecan were detected through immunohistochemistry and RT-PCR. <br> RESULTS AND CONCLUSION:The synovial mesenchymal stem cellpel ets cultured in the chondrocyte supernatant became cartilage-like tissue after 21 days. The type II col agen was detected positively in the matrix of synovial mesenchymal stem cellpel et immunohistochemical y. RT-PCR examination showed that the type II col agen and aggrecan expressed in the synovial mesenchymal stem cellpel et cultured in the chondrocyte supernatant. It suggested that synovial mesenchymal stem cellcould be induced to differentiate into chondrocytes depending on soluble factors secreted by chondrocytes.关键词
干细胞/诱导/滑膜间充质干细胞/软骨形成/软骨细胞/可溶性因子/生长因子/种子细胞/三维培养/新疆维吾尔自治区自然科学基金Key words
chondrocytes/synovial membrane/mesenchymal stem cells/cellculture/celldifferentiation/tissue engineering分类
医药卫生引用本文复制引用
邵博,林兆全,龚忠诚,刘慧,凌彬,克热木,尹小朋,胡露露,王冰,宁晓婷..软骨细胞上清液诱导滑膜间充质干细胞微团培养成软骨[J].中国组织工程研究,2014,(1):100-105,6.基金项目
新疆维吾尔自治区自然科学基金项目(2011211A068)，项目名称PLLA/PDLA三维支架与滑膜间充质干细胞构建颞下颌关节盘的实验研究@@@@the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No.2011211A068 （2011211A068）
