中国组织工程研究Issue(2)：193-198,6.DOI:10.3969/j.issn.2095-4344.2014.02.006
牙胚细胞体外培养过程中成牙基因的表达
Odontogenesis-related gene expression during in vitro culture of tooth germ cells
摘要
Abstract
BACKGROUND:Some studies have indicated that different genes in tooth germ tissue play a role at different time, contributing to tooth germ development. <br> OBJECTIVE:To observe the expressions of dentin matrix protein 1, enamel protein, col agen I and homeobox gene 1 at different stage of in vitro culture of tooth germ cells. <br> METHODS:RNA from tooth germ cells was extracted at days 1, 3, 6 of in vitro culture. After reverse transcription, real-time quantitative PCR detection was adopted to measure relative expression of dentin matrix protein 1, enamel protein, col agen I and homeobox gene 1 mRNA. <br> RESULTS AND CONCLUSION:Dentin matrix protein 1, enamel protein, and col agen I mRNA expressions increased with culture time, and reached the peak at day 3 (P<0.05), whereas homeobox gene 1 mRNA decreased with culture time (P<0.05).关键词
组织构建/组织工程/口腔组织工程/牙胚细胞/培养/牙本质基质蛋白1/成釉蛋白/Ⅰ型胶原蛋白/同源异型盒基因1Key words
cell culture techniques/tooth germ/gene expression/odontogenesis分类
医药卫生引用本文复制引用
巴娇娇,何惠宇,胡杨,马嵋,韩祥祯..牙胚细胞体外培养过程中成牙基因的表达[J].中国组织工程研究,2014,(2):193-198,6.基金项目
新疆维吾尔自治区科技厅科技支疆项目(201291173)@@@@Science and Technology Project of Science and Technology Department of Xinjiang Uygur Autonomous Region, No.201291173 （201291173）
