中国组织工程研究Issue(5)：663-668,6.DOI:10.3969/j.issn.2095-4344.2014.05.002
医用TH胶栓塞建立月骨缺血性坏死兔模型
Medical TH adhesive embolism for establishing a rabbit model of ischemic necrosis of lunate bone
摘要
Abstract
BACKGROUND:Kienböck disease lacks of suitable animal models, which are similar to the pathological process of avascular necrosis of human lunate bone. <br> OBJECTIVE:To establish a new animal model of Kienböck disease using medical TH adhesive embolism and to explore the rationality of model establishment. <br> METHODS:A total of 30 healthy adult New Zealand rabbits, male or female, were selected. Using self-control method, the rabbits were randomly assigned to experimental sides and control sides. By dril ing in the center of the lunate bone, 0.2 mL of medical TH glue was injected three times. An equal volume of physiological saline was injected into the center of the lunate bone on the control side. X-ray examination, general observation, Micro-CT measurement of bone, and tissue pathology detection were conducted at 4, 8 and 12 weeks. <br> RESULTS AND CONCLUSION:Gross specimen, X-ray and histological results showed that ischemic necrosis of the lunate bone on the experimental side was visible at 8 weeks after model induction. The ischemic necrosis of the lunate bone became more typical at 12 weeks. Among the Micro-CT microscopic parameters of trabecular bone, trabecular bone density parameters bone volume fraction and the number of trabecular bone were significantly lower on the experimental side than those on the control side (P<0.05). Spatial parameters of trabecular bone significantly increased. Trabecular separation and structure model index on the experimental side were significantly greater than those on the control side. Results suggested that ischemic necrosis of the lunate bone appeared on the experimental side at 8 weeks after injection of medical TH glue. Rabbit models of ischemic necrosis of the lunate bone can be established at 12 weeks. Thus, alterations, which were similar to ischemic necrosis of human lunate bone, appeared, such as blood transportation damage in the lunate bone, trabecular bone fracture, and empty lacuna, when surrounding tissues were not obviously injured.关键词
实验动物/组织构建/Kienböck病/月骨/动物模型/TH胶/骨小梁/Micro-CT/X射线Key words
models, animal/lunate bone/osteonecrosis/histology分类
医药卫生引用本文复制引用
路云翔,陈郁鲜,庄泽,任建华,彭优,史德海,王昆,李智勇..医用TH胶栓塞建立月骨缺血性坏死兔模型[J].中国组织工程研究,2014,(5):663-668,6.基金项目
广东省医学科学基金项目(A2012173)，课题名称骨髓间充质干细胞复合生物材料治疗月骨无菌性坏死 （A2012173）
