中国组织工程研究Issue(5)：736-741,6.DOI:10.3969/j.issn.2095-4344.2014.05.014
两种免疫法联合肾功能指标测定他克莫司血药浓度的比较
Comparative analysis of tacrolimus blood concentrations by two determination methods based on renal function
李想 1颜鸣 1史国兵 1夏东亚 1任天舒1
作者信息
- 1. 解放军沈阳军区总医院，辽宁省沈阳市 110840
- 折叠
摘要
Abstract
BACKGROUND:Whether determination of tacrolimus blood concentration by different immunoassay methods can influence predictive ability to immunosuppressive effects and toxicity, and whether it can be more sensitive to reflect blood concentration in patients with renal dysfunction are worthy of studying. <br> OBJECTIVE:To analyze the correlation of tacrolimus (FK506) concentrations determined by enzyme-multiplied immunoassay technique (EMIT) and enzyme linked immunosorbent assay (ELISA) in combination with renal function parameters. <br> METHODS:133 clinical blood samples were col ected. EMIT and ELISA techniques were used to determine the FK506 concentration. The correlation of two determination methods were analyzed, combined with renal function. RESULTS AND CONCLUSION:In patients with renal dysfunction, the mean results and standard deviation mensurated by ELISA were higher than those by EMIT. For blood concentration in 5-20μg/L by ELISA, the incidence of renal dysfunction occurred less than by EMIT. The overal mean results of blood concentration for two methods appeared no significant difference (r=0.904 5, P>0.05). When the concentration was less than 2.0μg/L, the concentration results by EMIT were higher than those by ELISA (P<0.01). When the concentration was more than 2.0μg/L, there was no significant difference between two determination methods (P>0.05). These findings indicate that EMIT and ELISA has good correlation, which are both suitable for clinical routine determination of plasma concentration. It is not recommended for applying EMIT method to determine low blood concentrations (<2.0μg/L). The reference range of concentration should be compartmentalized depending on combination of determination methods and renal function.关键词
实验动物/组织构建/肾移植/器官移植/他克莫司/酶联免疫吸附技术/酶增强免疫技术Key words
kidney transplantation/tacrolimus/enzyme-linked immunosorbent assay分类
医药卫生引用本文复制引用
李想,颜鸣,史国兵,夏东亚,任天舒..两种免疫法联合肾功能指标测定他克莫司血药浓度的比较[J].中国组织工程研究,2014,(5):736-741,6.
